INTERACTION BETWEEN CYTOCHROME C AND PURIFIED PHOSPHOLIPIDS

被引：27

作者：

HART, CJ

LESLIE, RB

DAVIS, MAF

LAWRENCE, GA

机构：

[1] Unilever Research Laboratory, Colworth Welwyn, Welwyn,, Herts Herts, The Frythe

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1969年 / 193卷 / 02期

关键词：

D O I：

10.1016/0005-2736(69)90191-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Isooctane-soluble complexes formed between cytochrome c and various mixtures of phosphatidylserine and phosphatidylcholine have been studied, with the following results. 1. 1. For a given phospholipid mixture there is a unique total lipid to protein ratio for maximum complex extraction. 2. 2. At maximum extraction, each cytochrome c molecule binds 9 molecules of phosphatidylserine and 5-60 molecules of phosphatidylcholine according to the composition of the original phospholipid mixture. When the phosphatidylserine to cytochrome c ratio exceeds 9:1, decreased extraction of both lipid and protein is observed. 3. 3. Other saturated paraffins exhibit similar bahaviour to isooctane, but unsaturated, or halogenated hydrocarbons, do not extract complexes under similar conditions. 4. 4. In 100 mM NaCl, each cytochrome c molecule is associated with 70-100 phosphatidylserine molecules, and a maximum of 400-500 phosphatidylcholine molecules. The results suggest an electrostatic interaction between the phosphatidylserine and protein. The mode of binding of the phosphatidylcholine is not clear. © 1969.

引用

页码：308 / &

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