ANTISENSE RNA PRODUCTION IN TRANSGENIC MICE

被引：28

作者：

MUNIR, MI ^{[1
]}

ROSSITER, BJF ^{[1
]}

CASKEY, CT ^{[1
]}

机构：

[1] BAYLOR UNIV,INST MOLEC GENET,HOUSTON,TX 77030

来源：

SOMATIC CELL AND MOLECULAR GENETICS | 1990年 / 16卷 / 04期

关键词：

D O I：

10.1007/BF01232466

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

There are many reports of antisense inhibition of gene expression in cultured cells. We have generated four strains of transgenic mice expressing antisense hypoxanthine guanine phosphoribosyltransferase (HPRT) RNA in brain, or heart and liver, or all three organs. In the brain of one strain, the level of antisense RNA in the different brain regions roughly correlates with the degree of inhibition of the native HPRT mRNA in those same regions. Despite this decrease of up to 60% of endogenous HPRT mRNA, no reproducible reduction in HPRT activity has been observed. Possible reasons for the differences between the effectiveness of antisense inhibition in cultured cells and transgenic animals are discussed. © 1990 Plenum Publishing Corporation.

引用

页码：383 / 394

页数：12

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