MICROTUBULAR TAU-PROTEIN AFTER COCAINE IN CULTURED SH-SY5Y HUMAN NEUROBLASTOMA

被引：9

作者：

LEW, GM

机构：

[1] Department of Anatomy, College of Human Medicine, Michigan State University, E. Lansing

来源：

GENERAL PHARMACOLOGY | 1992年 / 23卷 / 06期

关键词：

D O I：

10.1016/0306-3623(92)90295-U

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

1. SH-SY5Y, a human neuroblastoma cell line, was used as a tissue culture model to examine the hypothesis that cocaine may affect the metabolism of tau protein which stabilizes microtubules and promotes microtubule assembly. 2. Cocaine hydrochloride (10(-9)-10(-3) M) caused dose-dependent reductions in cell number, with 10(-3) M causing 28% reduction after 48 hr. 3. This drug also decreased tau protein (50 Kd) in the cytoplasmic (supernatant) as well as the membrane (pellet) fraction after 48-hr treatment.

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收藏

页码：1111 / 1113

页数：3

相关论文

共 23 条

[1] NEUROPSYCHOLOGICAL DEFICITS IN CHRONIC COCAINE ABUSERS [J].

ARDILA, A ;

ROSSELLI, M ;

STRUMWASSER, S .

INTERNATIONAL JOURNAL OF NEUROSCIENCE, 1991, 57 (1-2) :73-79

[2]

BIEDLER JL, 1973, CANCER RES, V33, P2643

[3] MODULATION OF COCAINE METABOLISM IN PRIMARY RAT HEPATOCYTE CULTURES - EFFECTS ON IRREVERSIBLE BINDING AND PROTEIN-BIOSYNTHESIS [J].

BOUIS, P ;

BOELSTERLI, UA .

TOXICOLOGY AND APPLIED PHARMACOLOGY, 1990, 104 (03) :429-439

[4]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[5] COCAINE STIMULATES RAT HYPOTHALAMIC CORTICOTROPIN-RELEASING HORMONE-SECRETION INVITRO [J].

CALOGERO, AE ;

GALLUCCI, WT ;

KLING, MA ;

CHROUSOS, GP ;

GOLD, PW .

BRAIN RESEARCH, 1989, 505 (01) :7-11

[6] COCAINE HEPATOTOXICITY IN CULTURED LIVER SLICES - A SPECIES COMPARISON [J].

CONNORS, S ;

RANKIN, DR ;

GANDOLFI, AJ ;

KRUMDIECK, CL ;

KOEP, LJ ;

BRENDEL, K .

TOXICOLOGY, 1990, 61 (02) :171-183

[7] ALZHEIMERS-DISEASE - TAU PROTEINS, THE PROMOTING FACTORS OF MICROTUBULE ASSEMBLY, ARE MAJOR COMPONENTS OF PAIRED HELICAL FILAMENTS [J].

DELACOURTE, A ;

DEFOSSEZ, A .

JOURNAL OF THE NEUROLOGICAL SCIENCES, 1986, 76 (2-3) :173-186

[8] TAU-PROTEIN FUNCTION IN LIVING CELLS [J].

DRUBIN, DG ;

KIRSCHNER, MW .

JOURNAL OF CELL BIOLOGY, 1986, 103 (06) :2739-2746

[9] DAILY COCAINE TREATMENT PRODUCES A PERSISTENT REDUCTION OF [H-3] DOPAMINE UPTAKE INVITRO IN RAT NUCLEUS-ACCUMBENS BUT NOT IN STRIATUM [J].

IZENWASSER, S ;

COX, BM .

BRAIN RESEARCH, 1990, 531 (1-2) :338-341

[10] KINETICS OF COCAINE IN HUMANS AFTER INTRAVENOUS AND INTRANASAL ADMINISTRATION [J].

JAVAID, JI ;

MUSA, MN ;

FISCHMAN, M ;

SCHUSTER, CR ;

DAVIS, JM .

BIOPHARMACEUTICS & DRUG DISPOSITION, 1983, 4 (01) :9-18