IDENTIFICATION OF SCYLLO-INOSITOL IN PROTON NMR-SPECTRA OF HUMAN BRAIN INVIVO

被引：78

作者：

MICHAELIS, T ^{[1
]}

HELMS, G ^{[1
]}

MERBOLDT, KD ^{[1
]}

HANICKE, W ^{[1
]}

BRUHN, H ^{[1
]}

FRAHM, J ^{[1
]}

机构：

[1] MAX PLANCK INST BIOPHYS CHEM,POSTFACH 2841,W-3400 GOTTINGEN,GERMANY

来源：

NMR IN BIOMEDICINE | 1993年 / 6卷 / 01期

关键词：

D O I：

10.1002/nbm.1940060116

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Scyllo-inositol has been identified in proton NMR spectra of mammalian brain in vitro and in vivo. In contrast to myo-inositol this isomer comprises six equivalent CH protons that yield a singlet resonance at a chemical shift of 3.35 ppm. 1-D and 2-D J-resolved proton NMR studies (7.O T) of perchloric acid extracts of brain tissues revealed different amounts of scyllo-inositol in man, sheep, cow and rat. Absolute quantification of localized short-echo time proton NMR spectra (2.0 T) of human brain in vivo resulted in scyllo-inositol concentrations of 0.35+/-0.06 mM for white matter (n=25), 0.43+/-0.11 mM for grey matter (n=23) and 0.57+/-0.14 mm for cerebellum (n=10). Evidence for a tight metabolic link to myo-inositol was supported by a simultaneous variation of myo- and scyllo-inositol in patients with various brain diseases.

引用

页码：105 / 109

页数：5

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