INCORPORATION OF (R) AND (S)-3',4'-SECO-THYMIDINE INTO OLIGODEOXYNUCLEOTIDES - HYBRIDIZATION PROPERTIES AND ENZYMATIC STABILITY

被引：41

作者：

NIELSEN, P

KIRPEKAR, F

WENGEL, J

机构：

[1] ODENSE UNIV,DEPT CHEM,DK-5230 ODENSE,DENMARK

[2] ODENSE UNIV,DEPT MOLEC BIOL,DK-5230 ODENSE,DENMARK

来源：

NUCLEIC ACIDS RESEARCH | 1994年 / 22卷 / 05期

关键词：

D O I：

10.1093/nar/22.5.703

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Novel flexible oligodeoxynucleotide analogues containing (R)- and (S)-3',4'-seco-thymidine were synthesized on an automated DNA-synthesizer using the phosphoramidite approach. Oligodeoxynucleotide analogues (17-mers) having one or three modifications in the middle or one or two modifications in the ends were evaluated with respect to hybridization properties and enzymatic stability. 3'-End-modified oligomers were stable towards 3'-exonuclease degradation and displayed acceptable hybridization properties.

引用

页码：703 / 710

页数：8

共 37 条

[21] SUGAR MODIFIED OLIGONUCLEOTIDES .1. CARBO-OLIGODEOXYNUCLEOTIDES AS POTENTIAL ANTISENSE AGENTS
PERBOST, M
LUCAS, M
CHAVIS, C
POMPON, A
BAUMGARTNER, H
RAYNER, B
GRIENGL, H
IMBACH, JL
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 165 (02) : 742 - 747
[22] POTENTIAL ANTIVIRAL AGENTS - STEREOSPECIFIC SYNTHESIS OF PURINES AND PYRIMIDINES SUBSTITUTED WITH CHIRAL ACYCLIC CHAINS BY SUGAR-RING OPENING OF ALPHA-L-ARABINOPYRANOSYL NUCLEOSIDES
PERIGAUD, C
GOSSELIN, G
IMBACH, JL
[J]. JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 1992, (15): : 1943 - 1952
[23] MATRIX-ASSISTED LASER-DESORPTION IONIZATION TIME-OF-FLIGHT MASS-SPECTROMETRY - A POWERFUL TOOL FOR THE MASS AND SEQUENCE-ANALYSIS OF NATURAL AND MODIFIED OLIGONUCLEOTIDES
PIELES, U
ZURCHER, W
SCHAR, M
MOSER, HE
[J]. NUCLEIC ACIDS RESEARCH, 1993, 21 (14) : 3191 - 3196
[24] 1-(2'-DEOXY-BETA-D-XYLOFURANOSYL)THYMINE BUILDING-BLOCKS FOR SOLID-PHASE SYNTHESIS AND PROPERTIES OF OLIGO(2'-DEOXYXYLONUCLEOTIDES)
ROSEMEYER, H
SEELA, F
[J]. HELVETICA CHIMICA ACTA, 1991, 74 (04) : 748 - 760
[25] Sambrook J., 1989, MOL CLONING LAB MANU
[26] C-1'-BRANCHED ACYCLOVIR DERIVATIVES - SYNTHESIS AND ANTIVIRAL EVALUATION
SCHEINER, P
GEER, A
BUCKNOR, AM
GADLER, H
PRICE, RW
[J]. NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS, 1989, 8 (08) : 1441 - 1451
[27] ACYCLIC ANALOGS OF 3'-AZIDO-3'-DEOXYTHYMIDINE AS POTENTIAL ANTIVIRAL AGENTS - NUCLEOSIDE SYNTHESIS BY MICHAEL ADDITION
SCHEINER, P
GEER, A
BUCKNOR, AM
IMBACH, JL
SCHINAZI, RF
[J]. JOURNAL OF MEDICINAL CHEMISTRY, 1989, 32 (01) : 73 - 76
[28] OLIGONUCLEOTIDES CONTAINING FLEXIBLE NUCLEOSIDE ANALOGS
SCHNEIDER, KC
BENNER, SA
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1990, 112 (01) : 453 - 455
[29] MODIFIED DEOXYOLIGONUCLEOTIDES STABLE TO EXONUCLEASE DEGRADATION IN SERUM
SHAW, JP
KENT, K
BIRD, J
FISHBACK, J
FROEHLER, B
[J]. NUCLEIC ACIDS RESEARCH, 1991, 19 (04) : 747 - 750
[30] NEW SYNTHETIC ROUTES TO PROTECTED PURINE 2'-O-METHYLRIBOSIDE-3'-O-PHOSPHORAMIDITES USING A NOVEL ALKYLATION PROCEDURE
SPROAT, BS
BEIJER, B
IRIBARREN, A
[J]. NUCLEIC ACIDS RESEARCH, 1990, 18 (01) : 41 - 49

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