DETERMINATION OF NEFAZODONE AND ITS METABOLITES IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH COULOMETRIC DETECTION

被引：13

作者：

FRANKLIN, M

机构：

[1] University of Oxford, Department of Psychiatry, Psychopharmacology Research Unit

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1993年 / 11卷 / 11-12期

关键词：

NEFAZODONE; METABOLITES; INTERNAL STANDARD; HPLC; COULOMETRIC DETECTION;

D O I：

10.1016/0731-7085(93)80090-N

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Nefazodone and its metabolites together with the internal standard are extracted from plasma using CN Bond-Elut sorbent columns. Chromatography and detection are performed using isocratic reversed-phase high-performance liquid chromatography (HPLC) with a CN column and coulometric end point detection. The lower limits of quantitation for nefazodone, mCPP, and OH-nefazodone was 0.9, 0.2 and 0.6 ng ml(-1) when 1 ml of plasma was extracted. The interassay relative standard deviations were less than 6% for nefazodone and its metabolites. Recovery averaged over 80%. Plasma profiles of nefazodone and its metabolites following oral dosing are presented.

引用

页码：1109 / 1113

页数：5

共 4 条

[1] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF NEFAZODONE AND ITS METABOLITES IN HUMAN PLASMA USING LABORATORY ROBOTICS [J].