MAPPING AND CHROMOSOME ANALYSIS - THE POTENTIAL OF FLUORESCENCE IN-SITU HYBRIDIZATION

被引：41

作者：

JOOS, S ^{[1
]}

FINK, TM ^{[1
]}

RATSCH, A ^{[1
]}

LICHTER, P ^{[1
]}

机构：

[1] DEUTSCH KREBSFORSCHUNGSZENTRUM,ORG KOMPLEXER GENOME ABT,D-69120 HEIDELBERG,GERMANY

来源：

JOURNAL OF BIOTECHNOLOGY | 1994年 / 35卷 / 2-3期

关键词：

FLUORESCENCE IN SITU HYBRIDIZATION; DIGITAL IMAGE MICROSCOPY; MULTICOLOR FISH; CHROMOSOMAL MAPPING; INTERPHASE CYTOGENETICS; COMPARATIVE GENOMIC HYBRIDIZATION;

D O I：

10.1016/0168-1656(94)90032-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Fluorescence in situ hybridization (FISH) is a method widely used for the delineation of chromosomal DNA. FISH is applied in many areas of basic research as well as in clinical cytogenetics. In this review important technical improvements as well as the various applications of this method are summarized. In the first part different labeling and detection procedures are described and the potential of various kinds of probes are discussed. Recent developments in optical instrumentation and digital imaging procedures are; outlined in the second part. The following important applications of FISH are discussed: (a) new strategies for high resolution mapping of DNA sequences; (b) detection of chromosomal aberrations in clinical material; (c) techniques allowing the simultaneous detection of numerous probes by multiple color FISH; and (d) the new approach of comparative genomic hybridization, allowing a rapid and comprehensive analysis of chromosomal imbalances in cell populations, which is particularly useful for the cytogenetic analysis of tumor samples.

引用

页码：135 / 153

页数：19

共 122 条

[41]

JOOS S, 1993, HUM GENET, V90, P584, DOI 10.1007/BF00202475

[42] ESTIMATION OF ANEUPLOIDY LEVELS IN HUMAN-SPERMATOZOA USING CHROMOSOME SPECIFIC PROBES AND INSITU HYBRIDIZATION [J].

JOSEPH, AM ;

GOSDEN, JR ;

CHANDLEY, AC .

HUMAN GENETICS, 1984, 66 (2-3) :234-238

[43] LUMINESCENCE DIGITAL IMAGING MICROSCOPY [J].

JOVIN, TM ;

ARNDTJOVIN, DJ .

ANNUAL REVIEW OF BIOPHYSICS AND BIOPHYSICAL CHEMISTRY, 1989, 18 :271-308

[44] COMPARATIVE GENOMIC HYBRIDIZATION FOR MOLECULAR CYTOGENETIC ANALYSIS OF SOLID TUMORS [J].

KALLIONIEMI, A ;

KALLIONIEMI, OP ;

SUDAR, D ;

RUTOVITZ, D ;

GRAY, JW ;

WALDMAN, F ;

PINKEL, D .

SCIENCE, 1992, 258 (5083) :818-821

[45]

KALLIONIEMI OP, 1993, SEMIN CANCER BIOL, V4, P41

[46]

KIBBELAAR RE, 1992, BLOOD, V79, P1823

[47] DIRECT NONRADIOACTIVE INSITU HYBRIDIZATION OF SOMATIC-CELL HYBRID DNA TO HUMAN LYMPHOCYTE CHROMOSOMES [J].

KIEVITS, T ;

DEVILEE, P ;

WIEGANT, J ;

WAPENAAR, MC ;

CORNELISSE, CJ ;

VANOMMEN, GJB ;

PEARSON, PL .

CYTOMETRY, 1990, 11 (01) :105-109

[48]

KLEVER M, 1991, HUM GENET, V86, P484

[49]

KLINGER K, 1992, AM J HUM GENET, V51, P55

[50] IMMUNOPHENOTYPING OF ANEUPLOID CELLS [J].

KNUUTILA, S ;

TEERENHOVI, L .

CANCER GENETICS AND CYTOGENETICS, 1989, 41 (01) :1-17

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