MOLECULAR CLONING OF POLYOMA-VIRUS DNA IN ESCHERICHIA-COLI - PLASMID VECTOR SYSTEM

被引：58

作者：

ISRAEL, MA ^{[1
]}

CHAN, HW ^{[1
]}

ROWE, WP ^{[1
]}

MARTIN, MA ^{[1
]}

机构：

[1] NIAID,VIRAL DIS LAB,BETHESDA,MD 20014

来源：

SCIENCE | 1979年 / 203卷 / 4383期

关键词：

D O I：

10.1126/science.217087

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A series of recombinant plasmids containing polyoma virus (PY) DNA were constructed, and their biological activity was evaluated in mice and in cultured mouse cells. While all of the recombinants studied contain the complete, potentially infectious viral DNA, in no case was the intact recombinant PY-plasmid DNA, or live Escherichia coli containing the recombinant plasmids, capable of inducing PY infection of mice, either by feeding or by parenteral injection.

引用

页码：883 / 887

页数：5

共 25 条

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[2] CONSTRUCTION AND CHARACTERIZATION OF NEW CLONING VEHICLES .2. MULTIPURPOSE CLONING SYSTEM
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