MOLECULAR CLONING OF POLYOMA-VIRUS DNA IN ESCHERICHIA-COLI - LAMBDA PHAGE VECTOR SYSTEM

被引：36

作者：

CHAN, HW

ISRAEL, MA

GARON, CF

ROWE, WP

MARTIN, MA

机构：

[1] NIAID,BIOL VIRUSES LAB,BETHESDA,MD 20014

[2] NIAID,VIRAL DIS LAB,BETHESDA,MD 20014

来源：

SCIENCE | 1979年 / 203卷 / 4383期

关键词：

D O I：

10.1126/science.217088

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The biological activity of recombinant phage and recombinant phage DNA containing monomeric or dimeric polyoma DNA inserts was examined in mice and cultured mouse cells. Recombinant preparations containing a single copy of viral DNA were invariably noninfectious; molecules containing a dimeric polyoma DNA insert were at least seven orders of magnitude less infectious than polyoma virions after parenteral inoculation. No infection was detected with any recombinant preparation after oral administration.

引用

页码：887 / 892

页数：6

共 11 条

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