CHROMOSOMAL ASSIGNMENT OF 46-BRAIN-CDNAS

被引：70

作者：

POLYMEROPOULOS, MH ^{[1
]}

XIAO, H ^{[1
]}

GLODEK, A ^{[1
]}

GORSKI, M ^{[1
]}

ADAMS, MD ^{[1
]}

MORENO, RF ^{[1
]}

FITZGERALD, MG ^{[1
]}

VENTER, JC ^{[1
]}

MERRIL, CR ^{[1
]}

机构：

[1] NINCDS,RECEPTOR BIOCHEM & MOLEC BIOL SECT,BETHESDA,MD 20892

来源：

GENOMICS | 1992年 / 12卷 / 03期

关键词：

D O I：

10.1016/0888-7543(92)90439-Y

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Expressed sequence tags (ESTs) have been obtained from several hundred brain cDNAs as an initial effort to characterize expressed brain genes. These ESTs will become tools for human genome mapping and they will also provide candidate causative genes for inherited disorders affecting the central nervous system. We have developed a procedure for the rapid chromosomal assignment of these ESTs: cDNA sequences are first analyzed by a computer program to determine regions likely not to be interrupted by introns in the genomic DNA. A pair of oligonucleotide primers is then designed to amplify this region by the polymerase chain reaction using DNA template from human-rodent somatic cell hybrid chromosomal panels. The chromosomal assignment of the cDNA is determined by studying the segregation of the amplified products in these panels. In this paper we describe the mappig of 46 brain ESTs, as well as observations on the amplification of rodent sequences. © 1992.

引用

页码：492 / 496

页数：5

共 23 条

[1] DEVELOPMENT OF HUMAN CHROMOSOME-SPECIFIC PCR PRIMERS FOR CHARACTERIZATION OF SOMATIC-CELL HYBRIDS [J].

ABBOTT, C ;

POVEY, S .

GENOMICS, 1991, 9 (01) :73-77

[2] COMPLEMENTARY-DNA SEQUENCING - EXPRESSED SEQUENCE TAGS AND HUMAN GENOME PROJECT [J].

ADAMS, MD ;

KELLEY, JM ;

GOCAYNE, JD ;

DUBNICK, M ;

POLYMEROPOULOS, MH ;

XIAO, H ;

MERRIL, CR ;

WU, A ;

OLDE, B ;

MORENO, RF ;

KERLAVAGE, AR ;

MCCOMBIE, WR ;

VENTER, JC .

SCIENCE, 1991, 252 (5013) :1651-1656

[3]

BOTSTEIN D, 1980, AM J HUM GENET, V32, P314

[4]

CALLEN DF, 1986, ANN GENET-PARIS, V29, P235

[5] CHROMOSOME LOCALIZATION OF HUMAN ARH GENES, A RAS-RELATED GENE FAMILY [J].

CANNIZZARO, LA ;

MADAULE, P ;

HECHT, F ;

AXEL, R ;

CROCE, CM ;

HUEBNER, K .

GENOMICS, 1990, 6 (02) :197-203

[6] APOLIPOPROTEIN-J - STRUCTURE AND TISSUE DISTRIBUTION [J].

DESILVA, HV ;

HARMONY, JAK ;

STUART, WD ;

GIL, CM ;

ROBBINS, J .

BIOCHEMISTRY, 1990, 29 (22) :5380-5389

[7] SYSTEMATIC SCREENING OF YEAST ARTIFICIAL-CHROMOSOME LIBRARIES BY USE OF THE POLYMERASE CHAIN-REACTION [J].

GREEN, ED ;

OLSON, MV .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (03) :1213-1217

[8] A SURVEY ON INTRON AND EXON LENGTHS [J].

HAWKINS, JD .

NUCLEIC ACIDS RESEARCH, 1988, 16 (21) :9893-9908

[9]

MADAULE P, 1985, CELL, V41, P37

[10] ASSIGNMENT OF HUMAN 3-HYDROXY-3-METHYLGLUTARYL COENZYME-A REDUCTASE GENE TO Q13-]Q23 REGION OF CHROMOSOME-5 [J].

MOHANDAS, T ;

HEINZMANN, C ;

SPARKES, RS ;

WASMUTH, J ;

EDWARDS, P ;

LUSIS, AJ .

SOMATIC CELL AND MOLECULAR GENETICS, 1986, 12 (01) :89-94

← 1 2 3 →