N-METHYL-D-ASPARTATE STIMULATION OF THE SURVIVAL OF RAT CEREBELLAR GRANULE CELLS IN CULTURE IS NOT DEPENDENT UPON INCREASED C-FOS EXPRESSION AND IS NOT MIMICKED BY PROTEIN-KINASE-C ACTIVATION

被引：19

作者：

GRAHAM, ME

BURGOYNE, RD

机构：

[1] Department of Physiology, University of Liverpool, Liverpool

来源：

NEUROSCIENCE LETTERS | 1991年 / 130卷 / 02期

基金：

英国惠康基金;

关键词：

C-FOS EXPRESSION; PHORBOL ESTER; PROTEIN KINASE-C; N-METHYL-D-ASPARTATE; CEREBELLAR GRANULE CELL;

D O I：

10.1016/0304-3940(91)90412-M

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The role of c-fos expression and protein kinase C in the survival of primary cultures of rat cerebellar granule cells was investigated. Results from immunocytochemistry and immunoblotting suggest that increased c-fos expression is not essential for the survival of cells grown in low K+ media in the presence of N-methyl-D-aspartate (NMDA) at the critical time point when sensitivity to survival requirements develops. In addition the phorbol ester, phorbol 12-myristate 13-acetate failed to bring about survival of cells cultured in low K+ media in the absence of NMDA when given chronically, suggesting that protein kinase C activation alone is not sufficient to maintain granule cell survival in culture.

引用

页码：267 / 270

页数：4

共 21 条

[11]

GALLO V, 1987, J NEUROSCI, V7, P2203

[12] SELECTIVE LOSS OF PURKINJE AND GRANULE CELL RESPONSIVENESS TO N-METHYL-D-ASPARTATE IN RAT CEREBELLUM DURING DEVELOPMENT [J].