SOLUTION STRUCTURE OF ENDOTHELIN-3 DETERMINED USING NMR-SPECTROSCOPY

被引:45
作者
MILLS, RG
ODONOGHUE, SI
SMITH, R
KING, GF
机构
[1] UNIV SYDNEY, DEPT BIOCHEM, SYDNEY, NSW 2006, AUSTRALIA
[2] UNIV QUEENSLAND, DEPT BIOCHEM, ST LUCIA, QLD 4072, AUSTRALIA
关键词
D O I
10.1021/bi00139a030
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The aqueous solution structure of the 21-residue vasoactive peptide hormone endothelin-3 has been determined using high-resolution NMR spectroscopy, A total of 177 proton-proton distance measurements and 5-chi-1 dihedral angle constraints derived from NMR spectra were used to calculate the structure using a combination of distance geometry and dynamical simulated annealing calculations. The calculations reveal a highly ordered, compact conformation in which a helical region extending from K9 to C15 lies in close apposition with the C-terminal hexapeptide; this interaction seems to be largely driven by hydrophobic interactions. Structure-activity studies are interpreted in terms of the conformational features of the calculated endothelin-3 structure.
引用
收藏
页码:5640 / 5645
页数:6
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