MUSCARINIC RECEPTOR-MEDIATED INCREASE IN ZETA-PKC EXPRESSION IN SK-N-SH HUMAN NEUROBLASTOMA-CELLS

被引：7

作者：

BAUMGOLD, J ^{[1
]}

DYER, KD ^{[1
]}

机构：

[1] GEORGE WASHINGTON UNIV,MED CTR,DEPT PHARMACOL,WASHINGTON,DC 20037

来源：

NEUROCHEMICAL RESEARCH | 1994年 / 19卷 / 02期

关键词：

PROTEIN KINASE C; MUSCARINIC RECEPTOR; NEUROBLASTOMA CELLS; WESTERN BLOT;

D O I：

10.1007/BF00966807

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Anti-peptide antibodies specific for each protein kinase C (PKC) isozyme were used to screen SK-N-SH human neuroblastoma cells. These cells were found to express only alpha- and zeta-PKC. Stimulation of these cells with phorbol esters caused alpha- but not zeta-PKC to translocate from cytosolic to membrane fractions. Stimulation of these cells with carbachol, which releases inositol trisphosphate and diacylglycerol, caused a transient translocation of alpha-PKC but not of zeta-PKC. Carbachol did, however, cause a gradual increase in immunoreactive zeta-PKC which reached maximal values 10-20 min after stimulation. These results implicate zeta-PKC in a receptor-mediated signalling event.

引用

页码：135 / 138

页数：4

共 17 条

[1] ACETYLCHOLINE ANALOG STIMULATES DNA-SYNTHESIS IN BRAIN-DERIVED CELLS VIA SPECIFIC MUSCARINIC RECEPTOR SUBTYPES
ASHKENAZI, A
RAMACHANDRAN, J
CAPON, DJ
[J]. NATURE, 1989, 340 (6229) : 146 - 150
[2] CALCIUM INDEPENDENCE OF PHOSPHOINOSITIDE HYDROLYSIS-INDUCED INCREASE IN CYCLIC-AMP ACCUMULATION IN SK-N-SH HUMAN NEUROBLASTOMA-CELLS
BAUMGOLD, J
PAEK, R
FISKUM, G
[J]. JOURNAL OF NEUROCHEMISTRY, 1992, 58 (05) : 1754 - 1759
[3] AGENTS THAT STIMULATE PHOSPHOINOSITIDE TURNOVER ALSO ELEVATE CAMP IN SK-N-SH HUMAN NEUROBLASTOMA-CELLS
BAUMGOLD, J
PAEK, R
YASUMOTO, T
[J]. LIFE SCIENCES, 1992, 50 (23) : 1755 - 1759
[4] PHARMACOLOGICAL DIFFERENCES BETWEEN MUSCARINIC RECEPTORS COUPLED TO PHOSPHOINOSITIDE TURNOVER AND THOSE COUPLED TO ADENYLATE-CYCLASE INHIBITION
BAUMGOLD, J
WHITE, T
[J]. BIOCHEMICAL PHARMACOLOGY, 1989, 38 (10) : 1605 - 1616
[5] STIMULATION OF ARACHIDONIC-ACID RELEASE AND INHIBITION OF MITOGENESIS BY CLONED GENES FOR MUSCARINIC RECEPTOR SUBTYPES STABLY EXPRESSED IN A9 L-CELLS
CONKLIN, BR
BRANN, MR
BUCKLEY, NJ
MA, AL
BONNER, TI
AXELROD, J
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (22) : 8698 - 8702
[6] EVIDENCE FOR A ROLE OF PROTEIN-KINASE-C ZETA-SUBSPECIES IN MATURATION OF XENOPUS-LAEVIS OOCYTES
DOMINGUEZ, I
DIAZMECO, MT
MUNICIO, MM
BERRA, E
DEHERREROS, AG
CORNET, ME
SANZ, L
MOSCAT, J
[J]. MOLECULAR AND CELLULAR BIOLOGY, 1992, 12 (09) : 3776 - 3783
[7] A PUTATIVE M3 MUSCARINIC CHOLINERGIC RECEPTOR OF HIGH MOLECULAR-WEIGHT COUPLES TO PHOSPHOINOSITIDE HYDROLYSIS IN HUMAN SK-N-SH NEURO-BLASTOMA CELLS
FISHER, SK
HEACOCK, AM
[J]. JOURNAL OF NEUROCHEMISTRY, 1988, 50 (03) : 984 - 987
[8] PROTEIN-KINASE-C GROUP-B MEMBERS PKC-DELTA, PKC-EPSILON, PKC-ZETA AND PKC-L(ETA) - COMPARISON OF PROPERTIES OF RECOMBINANT PROTEINS INVITRO AND INVIVO
LIYANAGE, M
FRITH, D
LIVNEH, E
STABEL, S
[J]. BIOCHEMICAL JOURNAL, 1992, 283 : 781 - 787
[9] NAKANISHI H, 1993, J BIOL CHEM, V268, P13
[10] THE MOLECULAR HETEROGENEITY OF PROTEIN KINASE-C AND ITS IMPLICATIONS FOR CELLULAR-REGULATION
NISHIZUKA, Y
[J]. NATURE, 1988, 334 (6184) : 661 - 665

← 1 2 →