MOLECULAR AND BIOLOGICAL CHARACTERIZATION OF FUSION REGULATORY PROTEINS (FRPS) - ANTI-FRP MABS INDUCED HIV-MEDIATED CELL-FUSION VIA AN INTEGRIN SYSTEM

被引：80

作者：

OHTA, H

TSURUDOME, M

MATSUMURA, H

KOGA, Y

MORIKAWA, S

KAWANO, M

KUSUGAWA, S

KOMADA, H

NISHIO, M

ITO, Y

机构：

[1] MIE UNIV, SCH MED, DEPT MICROBIOL, TSU, MIE 514, JAPAN

[2] KYUSHU UNIV, MED INST BIOREGULAT, DEPT IMMUNOL, HIGASHI KU, FUKUOKA 812, JAPAN

[3] NATL INST HLTH, DEPT ENTEROVIRUS, MUSASHIMURAYAMA, TOKYO 208, JAPAN

来源：

EMBO JOURNAL | 1994年 / 13卷 / 09期

关键词：

FUSION REGULATION; HIV; INTEGRIN;

D O I：

10.1002/j.1460-2075.1994.tb06479.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Anti-FRP mAbs induced polykaryocyte formation of U2ME-7 cells (CD4(+)U937 cells transfected with the HIV gp160 gene). Anti-FRP-1 mAb immunoprecipitated gp80-85, gp120 and homodimers of these peptides, and anti-FRP-2 mAb reacted with gp135 identically to the alpha 3 subunit of integrin. Both anti-FRP-1 and anti-FRP-2 mAb-induced cell fusion was blocked by anti-beta 1 integrin antibody, fibronectin or inhibiting anti-FRP-1 antibody. Therefore, anti-FRP mAbs were thought to induce the fusion via an integrin system(s). FRP-mediated fusion was temperature, cytoskeleton, energy and Ca2+ dependent. These experiments showed a possible regulatory function of cell fusion by an integrin system(s).

引用

页码：2044 / 2055

页数：12

共 40 条

[31] ROLE OF THE HTLV-III/LAV ENVELOPE IN SYNCYTIUM FORMATION AND CYTOPATHICITY [J].