A RAPID AND SENSITIVE IMMUNOASSAY FOR TUMOR-NECROSIS-FACTOR USING MAGNETIC MONODISPERSE POLYMER PARTICLES

被引:48
作者
LIABAKK, NB
NUSTAD, K
ESPEVIK, T
机构
[1] UNIV TRONDHEIM,INST CANC RES,TRONDHEIM,NORWAY
[2] NORWEGIAN RADIUM HOSP,CENT LAB,OSLO 3,NORWAY
关键词
Immunoassay; Magnetic monodisperse polymer particle; Tumor necrosis factor;
D O I
10.1016/0022-1759(90)90387-B
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A sensitive and rapid immunoassay for the detection of tumor necrosis factor (TNF) has been developed. Magnetic monodisperse polymer particles (M-280 Dynabeads) used as solid phase material, were coated with a neutralizing mouse monoclonal antibody to TNF. The coated Dynabeads were shown to have a more rapid binding capacity for recombinant (r) TNF as compared to standard immunowells coated with antibodies to TNF. The amount of TNF bound to the Dynabeads was quantified using either a polyclonal antibody to TNF or a mouse monoclonal antibody to TNF. The antibodies used for detection were either labelled with 125I or peroxidase. The linear assay range for the TNF standard curve was from 62 to 4000 pg/ml, and the assay time was less than 60 min. The sensitivity could be increased 5-8-fold by increasing the sample volume from 0.1 to 2 ml. © 1990.
引用
收藏
页码:253 / 259
页数:7
相关论文
共 24 条
  • [11] KRAMER SM, 1986, J IMMUNOL METHODS, V93, P210
  • [12] RAISED SERUM LEVELS OF CACHECTIN/TUMOR NECROSIS FACTOR-ALPHA IN RENAL-ALLOGRAFT REJECTION
    MAURY, CPJ
    TEPPO, AM
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1987, 166 (04) : 1132 - 1137
  • [13] Nustad K, 1984, Eur Surg Res, V16 Suppl 2, P80, DOI 10.1159/000128626
  • [14] HUMAN-TUMOR NECROSIS FACTOR - PRECURSOR STRUCTURE, EXPRESSION AND HOMOLOGY TO LYMPHOTOXIN
    PENNICA, D
    NEDWIN, GE
    HAYFLICK, JS
    SEEBURG, PH
    DERYNCK, R
    PALLADINO, MA
    KOHR, WJ
    AGGARWAL, BB
    GOEDDEL, DV
    [J]. NATURE, 1984, 312 (5996) : 724 - 729
  • [15] ELISA FOR QUANTITATION OF TUMOR NECROSIS FACTOR-ALPHA IN SERUM
    PRINCE, WS
    HARDER, KJ
    SAKS, S
    REED, BR
    CHEN, AB
    JONES, AJS
    [J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1987, 5 (08) : 793 - 802
  • [16] TUMOR NECROSIS FACTOR-ALPHA CACHECTIN IS A GROWTH-FACTOR FOR THYMOCYTES - SYNERGISTIC INTERACTIONS WITH OTHER CYTOKINES
    RANGES, GE
    ZLOTNIK, A
    ESPEVIK, T
    DINARELLO, CA
    CERAMI, A
    PALLADINO, MA
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1988, 167 (04) : 1472 - 1478
  • [17] ROBARD D, 1978, RADIOIMMUNOASSAY REL, V1, P469
  • [18] SCUDERI P, 1986, LANCET, V2, P1364
  • [19] SHALABY MR, 1988, J IMMUNOL, V141, P499
  • [20] RECOMBINANT HUMAN-TUMOR NECROSIS FACTOR-ALPHA - EFFECTS ON PROLIFERATION OF NORMAL AND TRANSFORMED-CELLS INVITRO
    SUGARMAN, BJ
    AGGARWAL, BB
    HASS, PE
    FIGARI, IS
    PALLADINO, MA
    SHEPARD, HM
    [J]. SCIENCE, 1985, 230 (4728) : 943 - 945