DETERMINATION OF AMINES BY FLOW-INJECTION ANALYSIS BASED ON ARYL OXALATE SULPHORHODAMINE-101 CHEMILUMINESCENCE

A flow-injection method for amines bawd On aryl oxalate-Sulphorhodamine 101 chemiluminescence is described. Fifty-five aliphatic, aromatic and heterocyclic amines were studied by the proposed method without a derivatization reaction or expensive apparatus. Twenty-eight amines were detected at levels of 1.0 x 10(-10)-4.0 x 10(-6) M (signal-to-noise ratio = 3, 20-mul injection) with linear calibration up to 1.0 x 10(-4)-1.0 x 10(-3) M, respectively. The relative standard deviation (n = 6) was 4.8% at 1.0 x 10(-6) M triethylamine. The proposed method was applied to the determination of histamine in fish and compared favourably with a liquid chromatographic method using fluorescence detection after derivatization.