EFFECT OF PH TEMPERATURE OSMOLARITY AND DRUGS ON 5-HYDROXYTRYPTAMINE CONTENT AND LIGHT AND ELECTRON MICROSCOPIC STRUCTURE OF RAT MAST CELLS IN VITRO

被引：14

作者：

JANSSON, SE

PENTTILA, A

机构：

来源：

EXPERIMENTAL CELL RESEARCH | 1969年 / 54卷 / 03期

关键词：

D O I：

10.1016/0014-4827(69)90216-X

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The effect of pH, temperature, osmolarity and of some drugs, commonly used in amine uptake and release experiments, on the 5-HT content of rat peritoneal mast cells was studied in vitro. The results were compared with morphological alterations of mast cells at light and electron microscopic level. The release of 5-HT increased, with the rise of temperature and hypotonicity of the incubation medium and with that of the incubation time. A pronounced release of 5-HT was found at pH below 4.0. The 5-HT release correlated well with degranulation and degenerative morphological changes of mast cells. The amine release induced by imipramine, chlorpromazine and Segontine® was dose-dependent. It was interpreted as a secondary phenomenon due to the destruction of the cells with drugs with subsequent leaking out of 5-HT. At room temperature reserpine caused only a slight release of 5-HT, but observed morphological changes were interpreted as a cause of a specific 5-HT release. © 1969.

引用

页码：367 / &

共 37 条

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