DUAL-DETECTOR POST-COLUMN REACTOR SYSTEM FOR THE DETECTION OF ISOENZYMES SEPARATED BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY .2. EVALUATION AND APPLICATION TO LACTATE-DEHYDROGENASE ISOENZYMES

被引：18

作者：

FULTON, JA ^{[1
]}

SCHLABACH, TD ^{[1
]}

KERL, JE ^{[1
]}

TOREN, EC ^{[1
]}

机构：

[1] UNIV S ALABAMA,DEPT PATHOL,MOBILE,AL 36688

来源：

JOURNAL OF CHROMATOGRAPHY | 1979年 / 175卷 / 02期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/S0021-9673(00)89434-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We describe the separation of lactate dehydrogenase isoenzymes by high-performance liquid chromatography-anion-exchange columns and their quantitation by a computer-controlled, dual-detector post-column reaction system. The recoveries from the separation column were ca. 90%. The dynamic range of the system was linear over about three orders of magnitude from 3 to 1500 U/l. The coefficient of variation for isoenzyme peak areas was ca. 2%. The method is compared to the classical electrophoresis measurement and shows increased speed, resolution, precision and accuracy. © 1979.

引用

页码：283 / 291

页数：9

共 12 条

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[4] DUAL-DETECTOR POST-COLUMN REACTOR SYSTEM FOR THE DETECTION OF ISOENZYMES SEPARATED BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY .1. DESCRIPTION AND THEORY
FULTON, JA
SCHLABACH, TD
KERL, JE
TOREN, EC
MILLER, AR
[J]. JOURNAL OF CHROMATOGRAPHY, 1979, 175 (02): : 269 - 281
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[7] REGNIER FE, Patent No. 4029583
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[9] ENZYME-SELECTIVE DETECTOR SYSTEMS FOR HIGH-PRESSURE LIQUID-CHROMATOGRAPHY
SCHROEDER, RR
KUDIRKA, PJ
TOREN, EC
[J]. JOURNAL OF CHROMATOGRAPHY, 1977, 134 (01): : 83 - 90
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