DUAL-DETECTOR POST-COLUMN REACTOR SYSTEM FOR THE DETECTION OF ISOENZYMES SEPARATED BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY .1. DESCRIPTION AND THEORY

We describe a dual-detector-post-column chromatographic reaction detector system that corrects for substances present in biological samples that interfere with the measurement of isoenzymes separated on a chromatographic column. The response observed at the detector in front of the reaction coil is mathematically dispersed, time transformed and subtracted from the detector behind the coil to produce a blank corrected chromatogram. The same computer program calculates peak areas and other chromatographic parameters such as height equivalent to a theoretical plate and retention time. In addition, we have evaluated the dispersion effects caused by various changes in our experimental system. © 1979.