GLYCOPROTEIN-III (CLUSTERIN, SULFATED GLYCOPROTEIN-2) IN ENDOCRINE, NERVOUS, AND OTHER TISSUES - IMMUNOCHEMICAL CHARACTERIZATION, SUBCELLULAR-LOCALIZATION, AND REGULATION OF BIOSYNTHESIS

被引：29

作者：

LASLOP, A

STEINER, HJ

EGGER, C

WOLKERSDORFER, M

KAPELARI, S

HOGUEANGELETTI, R

ERICKSON, JD

FISCHERCOLBRIE, R

WINKLER, H

机构：

[1] UNIV INNSBRUCK, DEPT PHARMACOL, PETER MAYR STR 1A, A-6020 INNSBRUCK, AUSTRIA

[2] YESHIVA UNIV ALBERT EINSTEIN COLL MED, DEPT DEV BIOL & CANC, BRONX, NY 10461 USA

[3] UNIV INNSBRUCK, DEPT PATHOL, A-6020 INNSBRUCK, AUSTRIA

[4] NIMH, CELL BIOL LAB, BETHESDA, MD 20892 USA

来源：

JOURNAL OF NEUROCHEMISTRY | 1993年 / 61卷 / 04期

关键词：

CLUSTERIN; LARGE DENSE-CORE VESICLES; ADRENAL MEDULLA; PITUITARY GLAND;

D O I：

10.1111/j.1471-4159.1993.tb13645.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Specific antisera were raised against the A and B chains of glycoprotein III. Immunoblotting revealed that in adrenal medulla both chains migrate very closely together in two-dimensional electrophoresis. Both chains with slightly differing molecular sizes are found in several endocrine tissues and in brain, kidney, liver, and serum. The mRNA has an analogous widespread distribution. In primary cultures of chromaffin cells the level of message becomes significantly increased by treatment with histamine or 12-O-tetradecanoylphorbol 13-acetate/forskolin. However, the increase is small when compared with that of secretogranin II. The subcellular localization of glycoprotein III in endocrine organs and in the posterior pituitary was investigated by subcellular fractionation and immunoelectron microscopy. Glycoprotein III was found to be confined to the large dense-core vesicles of these organs. For a discussion of the function of glycoprotein III, its localization in these organelles has to be taken into account.

引用

页码：1498 / 1505

页数：8