POLYMERIZATION OF THE BACTERIAL ELONGATION-FACTOR FOR PROTEIN-SYNTHESIS, EF-TU

被引：46

作者：

BECK, BD ^{[1
]}

机构：

[1] HARVARD UNIV,BIOL LABS,CAMBRIDGE,MA 02138

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1979年 / 97卷 / 02期

关键词：

D O I：

10.1111/j.1432-1033.1979.tb13137.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The bacterial elongation factor for protein synthesis, EF‐Tu, polymerizes into fibrils at pH 6.0. These fibrils are 0.7 μM in diameter, at least 200 μm in length, and are positively birefringent. Electron microscopic observations of negatively stained images demonstrates that the EF‐Tu fibrils consist of bundles of individual filaments, approximately 5 nm in diameter, aligned parallel to the long axis of the fibril. Polymerized EF‐Tu exchanges nucleotide rapidly and interacts with the other elongation factor, EF‐Ts. The antibiotic kirromycin induces the polymerization of EF‐Tu into fibrils and even larger structures under nonyolymerizing conditions. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：495 / 502

页数：8

共 52 条

[11] CELL LENGTH, CELL-GROWTH AND CELL-DIVISION
DONACHIE, WD
BEGG, KJ
VICENTE, M
[J]. NATURE, 1976, 264 (5584) : 328 - 333
[12] MEMBRANE STRUCTURES IN STABLE L-FORMS OF ESCHERICHIA-COLI
EDA, T
KANDA, Y
KIMURA, S
[J]. JOURNAL OF BACTERIOLOGY, 1976, 127 (03) : 1564 - 1567
[13] MICROTUBULAR STRUCTURES IN A STABLE STAPHYLOCOCCAL L-FORM
EDA, T
KANDA, Y
MORI, C
KIMURA, S
[J]. JOURNAL OF BACTERIOLOGY, 1977, 132 (03) : 1024 - 1026
[14] CONTENT OF ELONGATION-FACTOR TU IN ESCHERICHIA-COLI
FURANO, AV
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (12) : 4780 - 4784
[15] 2 CHROMATOGRAPHICALLY SEPARABLE FORMS OF ESCHERICHIA-COLI ELONGATION FACTOR-TU
GEISER, M
GORDON, J
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (03) : 1140 - 1144
[16] INHIBITION OF MYCOPLASMA CELL-DIVISION BY CYTOCHALASIN-B
GHOSH, A
MANILOFF, J
GERLING, DA
[J]. CELL, 1978, 13 (01) : 57 - 64
[17] A STEPWISE REACTION YIELDING A COMPLEX BETWEEN A SUPERNATANT FRACTION FROM E COLI GUANOSINE 5'-TRIPHOSPHATE AND AMINOACYL-SRNA
GORDON, J
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1968, 59 (01) : 179 - &
[18] GORDON J, 1969, J BIOL CHEM, V244, P5680
[19] PURIFICATION OF FACTOR TS - STUDIES ON FORMATION AND STABILITY OF NUCLEOTIDE COMPLEXES CONTAINING TRANSFER FACTOR TU
HACHMANN, J
MILLER, DL
WEISSBACH, H
[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1971, 147 (02) : 457 - +
[20] DETERMINATION OF PROTEIN - MODIFICATION OF LOWRY METHOD THAT GIVES A LINEAR PHOTOMETRIC RESPONSE
HARTREE, EF
[J]. ANALYTICAL BIOCHEMISTRY, 1972, 48 (02) : 422 - &

← 1 2 3 4 5 6 →