FURTHER EVIDENCE FOR THE ISOLATION OF XYLELLA-FASTIDIOSA ON NUTRIENT AGAR FROM GRAPEVINES SHOWING PIERCES DISEASE SYMPTOMS

被引：5

作者：

CHANG, CJ

ROBACKER, CD

LANE, RP

机构：

[1] Department of Plant Pathology, University of Georgia, Georgia Station, Griffin, GA

[2] Department of Horticulture, University of Georgia, Georgia Station, Griffin, GA

来源：

CANADIAN JOURNAL OF PLANT PATHOLOGY-REVUE CANADIENNE DE PHYTOPATHOLOGIE | 1990年 / 12卷 / 04期

关键词：

D O I：

10.1080/07060669009500981

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Nutrient agar (NA) was compared with CS20 and PD2 media for isolation of Xylella fastidiosa, the causal bacterium of Pierce's disease of grapevines. Branches showing Pierce's disease symptoms were collected from muscadine grapes (Vitis rotundifolia), American bunch grapes (Vitis labrusca), and French hybrids (Vitis vinifera x V. labrusca). Surface-sterilized petioles were comminuted in PD2 broth medium, and the resulting suspension was streaked onto all three media. NA as well as CS20 and PD2 media supported the primary isolation of X. fastidiosa. Strains isolated in NA medium were injected into healthy grapes and they induced typical symptoms as did those isolated in CS20 and PD2 media. The bacterium was reisolated from the inoculated plants, fulfilling Koch's postulates.

引用

页码：405 / 408

页数：4

共 17 条

[1]

Agrios G.N., Plant Pathology, (1988)

[2]

Auger J.G., Shalla T.A., Kado C.I., Pieree's disease of grapevines: Evidence for a bacterial etiology, Science, 184, pp. 1375-1377, (1974)

[3]

Chang C.J., Yonce C.E., Overwintering ol plum leal scald bacteria m infected trees, Ann. Phytopathol. Soc. Japan, 53, pp. 345-353, (1987)

[4]

Chang C.J., Walker J.T., Bacterial leat scorch of northern red oak: Isolation, cultivation, and pathogenicitv ol a xylcm-limued bacterium, Plant Dis, 72, pp. 730-733, (1988)

[5]

Davis M.J., Purcell A.H., Thomson S.Y., Pierces disease ot grapevines: Isolation of the causal bacterium, Science, 199, pp. 75-77, (1978)

[6]

Davis M.J., Purcell A.H., Thomson S.V., Isolation medium for the Pieree's disease bacterium, Phytopathology, 70, pp. 425-429, (1980)

[7]

Davis M.J., French W.J., Schaad N.W., Axcnic culture of the bacteria associated with phony disease of peach and plum leaf scald, Curr. Mierobiol, 6, pp. 309-314, (1981)

[8]

Davis M.J., Raju B.C., Brlansky R.H., Lee R.F., Timmer L.W., Norris R.C., McCoy R.E., Periwinkle wilt bacterium: Axenic culture, pathogenicitv. And relationships to other Gram-negative xylem-inhabiting bacteria, Phytopathology, 73, pp. 1510-1515, (1983)

[9]

Fry S.M., Milholland R.D., Huang P.-Y., Isolation and growth of the Pieree's disease bacterium on simple bacteriological media, Phytopathology, 78, (1988)

[10]

Fry S.M., Milholland R.D., Huang P.-Y., Isolation and growth of strains of Kylella fastidiosu from infected grapevines on nutrient agar media, Plant Dis, 74, pp. 522-524, (1990)

← 1 2 →