DENOVO SYNTHESIS AND DESATURATION OF FATTY-ACIDS AT THE MITOCHONDRIAL ACYL-CARRIER PROTEIN, A SUBUNIT OF NADH-UBIQUINONE OXIDOREDUCTASE IN NEUROSPORA-CRASSA

被引：48

作者：

ZENSEN, R ^{[1
]}

HUSMANN, H ^{[1
]}

SCHNEIDER, R ^{[1
]}

PEINE, T ^{[1
]}

WEISS, H ^{[1
]}

机构：

[1] MAX PLANCK INST COAL RES,W-4330 MULHEIM,GERMANY

来源：

FEBS LETTERS | 1992年 / 310卷 / 02期

关键词：

NADH; UBIQUINONE OXIDOREDUCTASE; COMPLEX-I; MITOCHONDRIAL FATTY ACID SYNTHESIS; CARDIOLIPIN;

D O I：

10.1016/0014-5793(92)81324-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cultivated the cel mutant of Neurospora crassa defective in cytosolic fatty acid synthesis with [2-C-14]malonate and found radioactivity covalently attached to the mitochondrial acyl-carrier protein (ACP), a subunit of the respiratory chain NADH:ubiquinone oxidoreductase. We purified the ACP by reverse-phase HPLC: the bound acyl groups were trans-esterified to methylesters and analyzed by gas chromatography. The saturated C6 to C18 fatty acids and oleic acid were detected. De novo synthesis and desaturation of fatty acids at the ACP subunit of NADH:ubiquinone oxidoreductase and use of the products of this mitochondrial synthetic pathway for cardiolipin synthesis is discussed.

引用

页码：179 / 181

页数：3