Fatty acid esters of 2-ethyl-1-hexanol were produced in a small pilot scale from rapeseed oil by Candida cylindracea lipase catalyzed transesterification (alcoholysis) without added solvent. Up to 90% conversion of rapeseed oil (97% of theoretical) was obtained in 8 h in 2 kg scale at 37-degrees C with 3.4% (w/w) lipase immobilized on an anion exchange resin Amberlite XAD-7, rapeseed oil:2-ethyl-1-hexanol substrate molar ratio of 2.8, and 3% (w/w) of added water.