AUTOMATED-DETERMINATION OF FREE PHENYTOIN IN HUMAN PLASMA WITH ONLINE EQUILIBRIUM DIALYSIS AND COLUMN-SWITCHING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：17

作者：

ANDRESEN, AT ^{[1
]}

RASMUSSEN, KE ^{[1
]}

RUGSTAD, HE ^{[1
]}

机构：

[1] NATL HOSP NORWAY,RIKSHOSP,DEPT CLIN PHARMACOL,N-0027 OSLO,NORWAY

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 621卷 / 02期

关键词：

D O I：

10.1016/0378-4347(93)80095-L

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Free phenytoin in human plasma was automatically determined by on-line equilibrium dialysis using the automated sequential trace enrichment of dialysate (ASTED) sample preparation system and HPLC. The dialysis cell was a modification of the cell supplied with the ASTED. Total phenytoin was analysed with the same analytical set-up and plasma protein binding was determined. Free phenytoin was determined in plasma from epileptic patients and the results were compared to those obtained by ultrafiltration. Automated determination of free and total phenytoin in plasma by the ASTED-HPLC combination was shown to be an accurate and reproducible method and the results in free phenytoin analyses were in agreement with those found with ultrafiltration. The sample throughput with the automated on-line combination of dialysis and column-switching HPLC was 75 samples in 24 h when the sample was dialysed at 37 degrees C.

引用

页码：189 / 198

页数：10

共 21 条

[1] AUTOMATED ONLINE DIALYSIS AND COLUMN-SWITCHING HPLC DETERMINATION OF FLUMEQUINE AND OXOLINIC ACID IN FISH LIVER
ANDRESEN, AT
RASMUSSEN, KE
[J]. JOURNAL OF LIQUID CHROMATOGRAPHY, 1990, 13 (20): : 4051 - 4065
[2] ONLINE DIALYSIS AND WEAK CATION-EXCHANGE ENRICHMENT OF DIALYSATE - AUTOMATED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF PHOLCODINE IN HUMAN PLASMA AND WHOLE-BLOOD
ANDRESEN, AT
KROGH, M
RASMUSSEN, KE
[J]. JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1992, 582 (1-2): : 123 - 130
[3] EVALUATION OF AN ULTRAFILTRATION FLUORESCENCE POLARIZATION IMMUNOASSAY FOR MONITORING UNBOUND PHENYTOIN
ARGYLE, JC
KINNIBURGH, DW
COSTA, R
JENNISON, T
[J]. THERAPEUTIC DRUG MONITORING, 1984, 6 (01) : 117 - 120
[4] AUTOMATED SEQUENTIAL TRACE ENRICHMENT OF DIALYSATES AND ROBOTICS - A TECHNIQUE FOR THE PREPARATION OF BIOLOGICAL SAMPLES PRIOR TO HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
COOPER, JDH
TURNELL, DC
GREEN, B
VERILLON, F
[J]. JOURNAL OF CHROMATOGRAPHY, 1988, 456 (01): : 53 - 69
[5] POTENTIAL USE OF MICRODIALYSIS IN PHARMACOKINETICS - A PROTEIN-BINDING STUDY
EKBLOM, M
LUNDQVIST, T
SJOBERG, P
HAMMARLUNDUDENAES, M
[J]. PHARMACEUTICAL RESEARCH, 1992, 9 (01) : 155 - 158
[6] SERUM AND PLASMA FOR TOTAL AND FREE ANTI-CONVULSANT DRUG ANALYSES - EFFECTS ON EMIT ASSAYS AND ULTRAFILTRATION DEVICES
GODOLPHIN, W
TREPANIER, J
FARRELL, K
[J]. THERAPEUTIC DRUG MONITORING, 1983, 5 (03) : 319 - 323
[7] MICRODIALYSIS SAMPLING FOR DETERMINATION OF PLASMA-PROTEIN BINDING OF DRUGS
HERRERA, AM
SCOTT, DO
LUNTE, CE
[J]. PHARMACEUTICAL RESEARCH, 1990, 7 (10) : 1077 - 1081
[8] ULTRAFILTRATION COMPARED WITH EQUILIBRIUM DIALYSIS IN THE DETERMINATION OF UNBOUND PHENYTOIN IN PLASMA
KOIKE, Y
MAGNUSSON, A
STEINER, E
RANE, A
SJOQVIST, F
[J]. THERAPEUTIC DRUG MONITORING, 1985, 7 (04) : 461 - 465
[9] LUND L, 1971, CLIN PHARM, V13, P196
[10] LUNDE PKM, 1970, CLIN PHARMACOL THER, V11, P846

← 1 2 3 →