STRUCTURE OF THE MURINE CD40 LIGAND GENE

被引：28

作者：

TSITSIKOV, EN

RAMESH, N

GEHA, RS

机构：

[1] CHILDRENS HOSP, DIV IMMUNOL, BOSTON, MA 02115 USA

[2] HARVARD UNIV, SCH MED, DEPT PEDIAT, BOSTON, MA 02115 USA

来源：

MOLECULAR IMMUNOLOGY | 1994年 / 31卷 / 12期

关键词：

CD40; LIGAND; GP39; GENOMIC CLONE; GENE STRUCTURE; PROMOTER SEQUENCES;

D O I：

10.1016/0161-5890(94)90009-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mouse CD40 ligand (CD40L) gene was cloned, sequenced and characterized. DNA sequence analysis showed that the CD40L gene comprises five exons and four intervening introns, spread over 13-14 kb of genomic DNA. The putative site for initiation of mRNA transcription was identified at 67 bp upstream of the translation initiation (ATG) codon. The nucleotide sequence of the 5'-flanking region of this gene revealed the presence of several regulatory regions including a TATA-like box, an Spl-like box and six potential NF-AT-like motifs. The 3'-untranslated region of the murine CD40L gene contained two ATTTA-elements which are thought to confer instability to the mRNA of many cytokines and two adjacent dinucleotide repeates, (CT)(25) and (CA)(45). These elements may play a role in the post-transcriptional regulation of CD40L gene expression.

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页码：895 / 900

页数：6

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