ISOLATION OF YEAST ARTIFICIAL CHROMOSOME CLONES FROM 54 POLYMORPHIC LOCI MAPPED WITH HIGH ODDS ON HUMAN-CHROMOSOME-4

被引：8

作者：

FAN, JB

DEYOUNG, J

LAGACE, R

LINA, RA

XU, ZD

MURRAY, JC

BUETOW, KH

WEISSENBACH, J

GOOLD, RD

COX, DR

MYERS, RM

机构：

[1] STANFORD UNIV,SCH MED,DEPT GENET,STANFORD,CA 94305

[2] STANFORD UNIV,DEPT GENET,CTR HUMAN GENOME MAPPING,PALO ALTO,CA 94304

[3] UNIV CALIF SAN FRANCISCO,SCH MED,CARDIOVASC RES INST,SAN FRANCISCO,CA 94143

[4] UNIV IOWA HOSP,DEPT PEDIAT,IOWA CITY,IA 52242

[5] FOX CHASE CANC CTR,DIV POPULAT SCI,PHILADELPHIA,PA 19111

[6] INST PASTEUR,CNRS,URA 1445,UNITE GENET MOLEC HUMAINE,F-75015 PARIS,FRANCE

来源：

HUMAN MOLECULAR GENETICS | 1994年 / 3卷 / 02期

关键词：

D O I：

10.1093/hmg/3.2.243

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We constructed a yeast artificial chromosome (YAC) framework map of human chromosome 4 by screening a YAC library with 63 polymorphic DNA markers located on the chromosome. These genetic markers are from two framework meiotic maps that had previously been constructed by two research groups, and are placed on the two maps with odds for their order of 1000:1 or greater. In addition to isolating and determining the sizes of 141 YAC clones for 54 of these markers, we combined the two framework meiotic maps to produce a single integrated map. These combined maps and the YAC clones provide a set of extended DNA loci ordered at high odds that can be used to isolate additional polymorphic loci and genes, and to serve as a framework for obtaining a higher resolution physical map of the chromosome.

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页码：243 / 246

页数：4

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