ATOMIC-FORCE MICROSCOPY OF SINGLE-STRANDED AND DOUBLE-STRANDED DEOXYRIBONUCLEIC-ACID

被引：47

作者：

THUNDAT, T

ALLISON, DP

WARMACK, RJ

DOKTYCZ, MJ

JACOBSON, KB

BROWN, GM

机构：

[1] OAK RIDGE NATL LAB,DIV BIOL,OAK RIDGE,TN 37831

[2] OAK RIDGE NATL LAB,DIV CHEM,OAK RIDGE,TN 37831

来源：

JOURNAL OF VACUUM SCIENCE & TECHNOLOGY A-VACUUM SURFACES AND FILMS | 1993年 / 11卷 / 04期

关键词：

D O I：

10.1116/1.578312

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

The atomic force microscope (AFM) was used to image double-stranded DNA and single-stranded DNA adsorbed onto mica modified by Ba (II). Images of double-stranded DNA show an open circular shape without drastic contortions, and contour lengths that measure within 7% of the calculated length. Images of single-stranded DNA show two distinct forms; compact open circles with ''nodes'' or lumps almost uniformly distributed are the most frequently observed while less abundant are highly elongated circles with a few nodes. Heating the single-stranded DNA sample and altering the solvent conditions prior to adsorption was found to increase the number of elongated circular molecules observed in the AFM images.

引用

页码：824 / 828

页数：5

共 29 条

[1] ALLISON D, UNPUB
[2] ATOMIC FORCE MICROSCOPE
BINNIG, G
QUATE, CF
GERBER, C
[J]. PHYSICAL REVIEW LETTERS, 1986, 56 (09) : 930 - 933
[3] CIRCULAR DNA-MOLECULES IMAGED IN AIR BY SCANNING FORCE MICROSCOPY
BUSTAMANTE, C
VESENKA, J
TANG, CL
REES, W
GUTHOLD, M
KELLER, R
[J]. BIOCHEMISTRY, 1992, 31 (01) : 22 - 26
[4] IMAGING CRYSTALS, POLYMERS, AND PROCESSES IN WATER WITH THE ATOMIC FORCE MICROSCOPE
DRAKE, B
PRATER, CB
WEISENHORN, AL
GOULD, SAC
ALBRECHT, TR
QUATE, CF
CANNELL, DS
HANSMA, HG
HANSMA, PK
[J]. SCIENCE, 1989, 243 (4898) : 1586 - 1589
[5] ATOMIC FORCE MICROSCOPY OF SINGLE-STRANDED AND DOUBLE-STRANDED DNA
HANSMA, HG
SINSHEIMER, RL
LI, MQ
HANSMA, PK
[J]. NUCLEIC ACIDS RESEARCH, 1992, 20 (14) : 3585 - 3590
[6] REPRODUCIBLE IMAGING AND DISSECTION OF PLASMID DNA UNDER LIQUID WITH THE ATOMIC FORCE MICROSCOPE
HANSMA, HG
VESENKA, J
SIEGERIST, C
KELDERMAN, G
MORRETT, H
SINSHEIMER, RL
ELINGS, V
BUSTAMANTE, C
HANSMA, PK
[J]. SCIENCE, 1992, 256 (5060) : 1180 - 1184
[7] PROGRESS IN SEQUENCING DEOXYRIBONUCLEIC-ACID WITH AN ATOMIC FORCE MICROSCOPE
HANSMA, HG
WEISENHORN, AL
GOULD, SAC
SINSHEIMER, RL
GAUB, HE
STUCKY, GD
ZAREMBA, CM
HANSMA, PK
[J]. JOURNAL OF VACUUM SCIENCE & TECHNOLOGY B, 1991, 9 (02): : 1282 - 1284
[8] IMAGING AND NANODISSECTION OF INDIVIDUAL SUPERCOILED PLASMIDS BY ATOMIC FORCE MICROSCOPY
HENDERSON, E
[J]. NUCLEIC ACIDS RESEARCH, 1992, 20 (03) : 445 - 447
[9] LINDSAY SM, 1992, P SOC PHOTO-OPT INS, V1639, P84, DOI 10.1117/12.58177
[10] STM AND AFM IMAGES OF NUCLEOSOME DNA UNDER WATER
LINDSAY, SM
NAGAHARA, LA
THUNDAT, T
KNIPPING, U
RILL, RL
DRAKE, B
PRATER, CB
WEISENHORN, AL
GOULD, SAC
HANSMA, PK
[J]. JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 1989, 7 (02) : 279 - 287

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