ISOLATION OF A RAT AMILORIDE-BINDING PROTEIN CDNA CLONE - TISSUE DISTRIBUTION AND REGULATION OF EXPRESSION

被引:9
作者
VERITY, K [1 ]
FULLER, PJ [1 ]
机构
[1] MONASH MED CTR, PRINCE HENRYS INST MED RES, CLAYTON, VIC 3168, AUSTRALIA
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1994年 / 266卷 / 06期
关键词
SODIUM CHANNEL; ALDOSTERONE; SEMINAL VESICLE; CORTICOSTEROIDS;
D O I
10.1152/ajpcell.1994.266.6.C1505
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Sodium transport across high resistance epithelia involves bath an apical amiloride-sensitive sodium channel and the basal Na+-K+-ATPase pump. Aldosterone regulates sodium transport by increasing the sodium permeability of the sodium channels. To study further the regulation of gene expression in sodium-transporting epithelia by corticosteroids, we have cloned an amiloride-binding protein (ABP) cDNA from rat descending colon and kidney. Identical 311 nucleotide cDNAs were amplified from both rat descending colon and kidney, and the predicted amino acid sequence exhibited 83% homology to the equivalent region of the human peptide sequence. Use of this cDNA as a probe resulted in detection of a transcript in both the small and large bowel, thymus, and seminal vesicle. The latter tissue exhibited the highest level of rat ABP expression. Low to undetectable levels of rat ABP were expressed in the descending colon and kidney. No regulation of rat ABP by either class of corticosteroids was observed. Levels of ABP were low at birth and increased gradually to adult levels just before weaning in the bowel. The distribution of rat ABP is not as would be predicted for an aldosterone-induced gene and is thus unlikely to be a component of the amiloride-sensitive electrogenic sodium channel.
引用
收藏
页码:C1505 / C1512
页数:8
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