AMPEROMETRIC DETERMINATION OF PHOSPHATE BY USE OF A NUCLEOSIDE PHOSPHORYLASE XANTHINE-OXIDASE ENZYME SENSOR BASED ON A CLARK-TYPE HYDROGEN-PEROXIDE OR OXYGEN-ELECTRODE

被引：29

作者：

HAEMMERLI, SD

SULEIMAN, AA

GUILBAULT, GG

机构：

[1] University of New Orleans, Department of Chemistry, New Orleans

来源：

ANALYTICAL BIOCHEMISTRY | 1990年 / 191卷 / 01期

关键词：

D O I：

10.1016/0003-2697(90)90395-P

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A bienzymatie sensor for the determination of phosphate was constructed by coimmobilization of xanthine oxidase (EC 1.2.3.22) and nucleoside phosphorylase (EC 2.4.2.1) on a polycarbonate membrane mounted on the tips of amperometric hydrogen peroxide and oxygen electrodes. The sensor response was linear to phosphate concentrations in the range 10-250 μm. © 1990.

引用

页码：106 / 109

页数：4

共 12 条

[1]

DEGROOT H, 1985, BIOCHEM J, V229, P255

[2]

DWENGER A, 1985, METHOD ENZYMAT AN, V7, P382

[3] PHOSPHATE DETERMINATION BY ENZYMATIC COLORIMETRIC ASSAY [J].