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RESOLUTION OF DIHYDROXYEICOSANOATES AND OF DIHYDROXYEICOSATRIENOATES BY CHIRAL PHASE CHROMATOGRAPHY

被引：17
作者：
CAPDEVILA, JH
WEI, SZ
KUMAR, A
KOBAYASHI, J
SNAPPER, JR
ZELDIN, DC
BHATT, RK
FALCK, JR
机构：
[1] UNIV TEXAS, SW MED CTR, DEPT MOLEC GENET, DALLAS, TX 75235 USA
[2] VANDERBILT UNIV, MED CTR, SCH MED, DEPT MED, NASHVILLE, TN 37232 USA
[3] VANDERBILT UNIV, MED CTR, SCH MED, DEPT BIOCHEM, NASHVILLE, TN 37232 USA
来源：
ANALYTICAL BIOCHEMISTRY | 1992年 / 207卷 / 02期
关键词：
D O I：
10.1016/0003-2697(92)90006-S
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A chromatographic method is described for the direct enantiomeric characterization of 5,6-, 8,9-, 11,12-, and 14,15-vic-dihydroxyeicosatrienoic acids (DHETs), metabolites of the cytochrome P-450 arachidonate epoxygenase pathway, and of their corresponding saturated vic-dihydroxyeicosanoic acids. Following esterification, the individual methyl or pentafluorobenzyl esters are resolved by chiral-phase chromatography utilizing a Chiralcel OC or OD column. This methodology will find analytical and preparative applications since it is simple and efficient and preserves, intact, the diol functionality. © 1992.
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页码：236 / 240
页数：5
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