CONSECUTIVE DEMONSTRATION OF CATECHOLAMINES AND DOPAMINE-BETA-HYDROXYLASE WITHIN THE SAME SPECIMEN

被引：31

作者：

KONIG, R

机构：

[1] Anatomisches Institut der Universität, Heidelberg, D-6900

来源：

HISTOCHEMISTRY | 1979年 / 61卷 / 03期

关键词：

D O I：

10.1007/BF00508451

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A combined method was developed for characterization and differentiation of catecholamines in neuron populations containing more than one catecholamine, e.g. dopamine and norepinephrine. Its application to small intensely fluorescent (SIF-) cell clusters in sympathetic ganglia allows the successive demonstration of glyoxylic acid-induced catecholamine fluorescence and dopamine-β-hydroxylase by indirect immunofluorescence within the same tissue section. Applying this technique to an example, two types of SIF-cells were demonstrated in the guinea pig superior cervical ganglion. © 1979 Springer-Verlag.

引用

收藏

页码：301 / 305

页数：5

相关论文

共 25 条

[1] GLYOXYLIC ACID CONDENSATION - NEW FLUORESCENCE METHOD FOR HISTOCHEMICAL DEMONSTRATION OF BIOGENIC MONOAMINES [J].

AXELSSON, S ;

BJORKLUND, A ;

FALCK, B ;

LINDVALL, O ;

SVENSSON, LA .

ACTA PHYSIOLOGICA SCANDINAVICA, 1973, 87 (01) :57-62

[2] FLUORESCENCE HISTOCHEMISTRY OF BIOGENIC MONOAMINES - STUDY OF CAPACITY OF VARIOUS CARBONYL-COMPOUNDS TO FORM FLUOROPHORES WITH BIOGENIC MONOAMINES IN GAS-PHASE REACTIONS [J].

AXELSSON, S ;

BJORKLUND, A ;

LINDVALL, O .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1972, 20 (06) :435-+

[3] HISTOCHEMICAL AND BIOCHEMICAL CHARACTERIZATION OF RAT PARACERVICAL GANGLION [J].

BAKER, HA ;

BURKE, JP ;

BHATNAGAR, RK ;

VANORDEN, DE ;

VANORDEN, LS ;

HARTMAN, BK .

BRAIN RESEARCH, 1977, 132 (03) :393-405

[4] MECHANISMS OF FLUOROPHORE FORMATION IN HISTOCHEMICAL GLYOXYLIC ACID METHOD FOR MONOAMINES [J].

BJORKLUND, A ;

SVENSSON, LA ;

LINDVALL, O .

HISTOCHEMIE, 1972, 32 (02) :113-+

[5] RAPID, SIMPLE AND SENSITIVE METHOD FOR DEMONSTRATION OF CENTRAL CATECHOLAMINE-CONTAINING NEURONS AND AXONS BY GLYOXYLIC ACID-INDUCED FLUORESCENCE .2. DETAILED DESCRIPTION OF METHODOLOGY [J].

BLOOM, FE ;

BATTENBERG, ELF .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1976, 24 (04) :561-571

[6] MONOAMINE-CONTAINING PARANEURONS IN SYMPATHETIC-GANGLIA OF MAMMALS [J].

CHIBA, T .

ARCHIVUM HISTOLOGICUM JAPONICUM, 1977, 40 :163-176

[7] FLUORESZENZMETHODEN ZUR HISTOCHEMISCHEN SICHTBARMACHUNG VON MONOAMINEN .2. IDENTIFIZIERUNG DES FLUORESZIERENDEN PRODUKTES AUS DOPAMIN UND FORMALDEHYD [J].

CORRODI, H ;

HILLARP, NA .

HELVETICA CHIMICA ACTA, 1964, 47 (03) :911-&

[8] METHODOLOGICAL APPROACH TO RAPID AND SENSITIVE MONOAMINE HISTOFLUORESCENCE USING A MODIFIED GLYOXYLIC-ACID TECHNIQUE - SPG METHOD [J].

DELATORRE, JC ;

SURGEON, JW .

HISTOCHEMISTRY, 1976, 49 (02) :81-93

[9] FLUORESCENCE MICROSCOPICAL, IMMUNOHISTOCHEMICAL AND ULTRASTRUCTURAL STUDIES ON SYMPATHETIC-GANGLIA OF GUINEA-PIG, WITH SPECIAL REFERENCE TO SIF CELLS AND THEIR CATECHOLAMINE CONTENT [J].

ELFVIN, LG ;

HOKFELT, T ;

GOLDSTEIN, M .

JOURNAL OF ULTRASTRUCTURE RESEARCH, 1975, 51 (03) :377-396

[10] SMALL INTENSELY FLUORESCENT (SIF) CELLS AND NERVOUS TRANSMISSION IN SYMPATHETIC-GANGLIA [J].

ERANKO, O .

ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY, 1978, 18 :417-430