APPLICABILITY OF NEW CHIRAL STATIONARY PHASES IN THE SEPARATION OF RACEMIC PHARMACEUTICAL COMPOUNDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

The potential of contemporary chiral liquid chromatographic columns for the enantioseparation of racemates of pharmaceutical compounds was studied. Sixteen Organon compounds were selected, mostly cardiovascular or CNS-active drugs. Seven chiral stationary phases were used, viz., five different cellulose derivatives, an alpha-1-acid glycoprotein and a polymethacrylate phase, all coated on silica particles. A good enantioseparation, with a resolution higher than 1.0, was achieved for fifteen of the sixteen racemates. The best results were obtained on a Chiralcel OJ column, on which seven enantiomers were separated. With respect to the chromatographic performance, stability and/or selectivity, the cellulose derivatives (Chiralcel columns) were preferred over the protein and polymethacrylate columns.