EFFECT OF HORMONAL AND NEURONAL AGENTS ON ADENYLATE-CYCLASE FROM SMOOTH-MUSCLE OF THE GASTRIC ANTRUM

被引：9

作者：

BAUR, S ^{[1
]}

GRANT, B ^{[1
]}

SPAULDING, RK ^{[1
]}

机构：

[1] VET ADM HOSP,PALO ALTO,CA 94304

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 584卷 / 03期

基金：

美国国家卫生研究院;

关键词：

(Gastric antrum muscle); Adenylate cyclase; Hormone effect; Neuronal effect;

D O I：

10.1016/0304-4165(79)90113-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Smooth muscle adenylate cyclase of a membrane preparation of canine gastric antrum has been characterized, and the effect of hormonal and neuronal agents examined. The enzyme is active in the presence of Mg2+ or Mn2+, but is inhibited by Ca2+. The Km is 0.5 mM ATP, similar to the Km of skeletal muscle adenylate cyclase. The enzyme is activated by isoproterenol but not norepinephrine, consistent with a β2-catecholamine receptor-adenylate cyclase interaction. Secretin activates the enzyme in concentrations as low as 1 · 10-11 M, while glucagon was effective only at 1 · 10-6 M. Prostaglandin E1 and E2 have a biphasic effect with activation of adenylate cyclase at 1 · 10-5 M and a small but significant inhibition of enzyme activity at 1 · 10-11 M. © 1979.

引用

页码：365 / 374

页数：10