A LUCIFERASE EXPRESSION SYSTEM FOR PHYSARUM THAT FACILITATES ANALYSIS OF REGULATORY ELEMENTS

被引：12

作者：

BAILEY, J

BENARD, M

BURLAND, TG

机构：

[1] LAB BIOL & ULTRASTRUCT NOYAU,CNRS,UPR 272,F-94801 VILLEJUIF,FRANCE

[2] UNIV WISCONSIN,MCARDLE LAB CANC RES,MADISON,WI 53706

来源：

CURRENT GENETICS | 1994年 / 26卷 / 02期

基金：

英国惠康基金;

关键词：

TRANSIENT EXPRESSION; LUCIFERASE; PHYSARUM POLYCEPHALUM; PROTIST;

D O I：

10.1007/BF00313799

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have developed a transient expression system for the protist Physarum polycephalum based on firefly luciferase. We demonstrate the utility of this system for comparing the activities of different promoters in Physarum amoebae, and also for detecting genetic elements that affect the level of gene expression. This system is likely to facilitate improvements in the stable transformation of this organism.

引用

收藏

页码：126 / 131

页数：6

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