A NEW GASEOUS IMAGING DETECTOR FOR THE ASSAY OF LYMPHOCYTE-CULTURES

被引:3
作者
BATEMAN, JE [1 ]
JOYCE, A [1 ]
KNIGHT, SC [1 ]
BEDFORD, P [1 ]
机构
[1] CLIN RES CTR,HARROW HA1 3UJ,MIDDX,ENGLAND
关键词
D O I
10.1016/0168-9002(91)91058-4
中图分类号
TH7 [仪器、仪表];
学科分类号
0804 ; 080401 ; 081102 ;
摘要
Tritium-labelled cell cultures used in studies of lymphocyte proliferation at the Clinical Research Centre are blotted in arrays of 10 x 6 spots spaced at 6 mm. An imaging detector based on the differential induction signals produced at a central amplifying electrode has been developed for the imaging and assay of these blots. A spatial resolution approximately 2.5 mm FWHM attained over the aperture of 60 mm x 36 mm enables the individual spots to be reliably counted. Data is captured in a PC/AT at rates which permit an assay to be completed in typically 30-60 min. The simplicity of both the detector and the readout electronics leads to a low cost system. Images and assay results are presented.
引用
收藏
页码:354 / 358
页数:5
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