KINETIC-STUDY OF HYBRIDOMA METABOLISM AND ANTIBODY-PRODUCTION IN CONTINUOUS-CULTURE USING SERUM-FREE MEDIUM

被引：23

作者：

KUROKAWA, H ^{[1
]}

OGAWA, T ^{[1
]}

KAMIHIRA, M ^{[1
]}

PARK, YS ^{[1
]}

IIJIMA, S ^{[1
]}

KOBAYASHI, T ^{[1
]}

机构：

[1] NAGOYA UNIV,FAC ENGN,DEPT BIOTECHNOL,FURO CHO,CHIKUSA KU,NAGOYA 46401,JAPAN

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1993年 / 76卷 / 02期

关键词：

D O I：

10.1016/0922-338X(93)90069-K

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Effects of glucose and glutamine on metabolism and antibody production were investigated in continuous culture of hybridoma cells using a serum-free medium. Viable cell concentration remained virtually constant during culture. The production of inhibitory metabolites, such as lactate and ammonium, could be reduced by keeping the concentration of glucose or glutamine in the medium at a low level. The specific production rate of antibody at a low glucose or glutamine concentration was 3-fold higher than that at high concentrations of glucose and glutamine (greater than 2.5 mM and 1.0 mM, respectively). The results obtained in this study suggest that maintaining the concentrations of glucose and glutamine at low levels in a hybridoma culture is important for reducing the formation of inhibitory metabolites and to maintain high antibody productivity.

引用

页码：128 / 133

页数：6

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