EFFECT OF OLEIC-ACID ON HUMAN EPIDERMIS - FLUORESCENCE SPECTROSCOPIC INVESTIGATION

被引：18

作者：

GARRISON, MD ^{[1
]}

DOH, LM ^{[1
]}

POTTS, RO ^{[1
]}

ABRAHAM, W ^{[1
]}

机构：

[1] CYGNUS THERAPEUT SYST,REDWOOD CITY,CA 94063

来源：

JOURNAL OF CONTROLLED RELEASE | 1994年 / 31卷 / 03期

关键词：

FLUORESCENCE SPECTROSCOPY; OLEIC ACID; PENETRATION ENHANCEMENT; TRANSDERMAL DRUG DELIVERY; STRATUM CORNEUM;

D O I：

10.1016/0168-3659(94)90008-6

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The effect of oleic acid on the human epidermis was studied using phase-modulated fluorescence spectroscopy. Fluorescence lifetime and limiting anisotropy of a membrane fluorophore 1,6-diphenyl-1,3,5-hexatriene were measured in human epidermis treated with donor formulations containing 0-5% oleic acid. Transport of benzoic acid across human epidermis was also measured under these conditions. The flux enhancement, changes in the spectral parameters, and uptake of oleic acid into epidermis showed a saturation effect with increasing OA concentration in the formulation. There was a strong correlation between the flux enhancement and the fluorescence lifetime and limiting anisotropy. The present study demonstrates the use of fluorescence spectroscopy to follow the effect of a chemical perturbant on skin structure.

引用

页码：263 / 269

页数：7

共 24 条

[1] MEMBRANE STRUCTURAL DOMAINS - RESOLUTION LIMITS USING DIPHENYLHEXATRIENE FLUORESCENCE DECAY [J].