A SMALL GENE, DESIGNATED COMS, LOCATED WITHIN THE CODING REGION OF THE 4TH AMINO ACID-ACTIVATION DOMAIN OF SRFA, IS REQUIRED FOR COMPETENCE DEVELOPMENT IN BACILLUS-SUBTILIS

被引：135

作者：

HAMOEN, LW ^{[1
]}

ESHUIS, H ^{[1
]}

JONGBLOED, J ^{[1
]}

VENEMA, G ^{[1
]}

VANSINDEREN, D ^{[1
]}

机构：

[1] UNIV GRONINGEN,DEPT GENET,9751 NN HAREN,NETHERLANDS

来源：

MOLECULAR MICROBIOLOGY | 1995年 / 15卷 / 01期

关键词：

D O I：

10.1111/j.1365-2958.1995.tb02220.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The valine-activation domain-encoding portion of the srfA locus (srfA-d4) is not only involved in the non-ribosomal synthesis of surfactin, but is also required for the regulation of competence development. In this study we show that impairment of the adenylation activity of the valine-activating domain did not affect competence development. Deletion analysis and complementation studies delineated the competence-required portion of srfA-d4 to a 168 bp fragment, which contains a small open reading frame (ORF), designated comS, encoding a polypeptide of 46 amino acids, embedded within, but translated in, a frame different from that of srfA-d4. Introduction of an amber mutation in the comS-coding frame prevented competence development, demonstrating the involvement of comS in this prokaryotic specialization process.

引用

页码：55 / 63

页数：9