A MONOCLONAL-ANTIBODY IDENTIFIES VIMENTIN FILAMENTS IN SERTOLI CELLS AND IN A SUBSET OF EPITHELIAL-CELLS IN THE RAT EPIDIDYMIS, URINARY-BLADDER AND PROSTATE

被引：2

作者：

DANAHEY, DG

WU, JC

LIN, LH

DEPHILIP, RM

机构：

[1] OHIO STATE UNIV,DEPT CELL BIOL NEUROBIOL & ANAT,COLUMBUS,OH 43210

[2] OHIO STATE UNIV,DEPT OTOLARYNGOL,COLUMBUS,OH 43210

来源：

JOURNAL OF UROLOGY | 1995年 / 154卷 / 06期

关键词：

IMMUNOHISTOCHEMISTRY; INTERMEDIATE FILAMENTS; ANTIBODIES; MONOCLONAL; VIMENTIN;

D O I：

10.1016/S0022-5347(01)66726-0

中图分类号：

R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

1002 ; 100201 ;

摘要：

Purpose: We describe a monoclonal antibody (Mab B2) against vimentin filaments in rat Sertoli cells and in a subset of epithelial cells in the rat epididymis, prostate and urinary bladder. Materials and Methods: The immunoreactivity of Mab B2 was examined on cryostat sections and immunoblots and compared with the reactivity of a previously characterized monoclonal antibody against vimentin. Results: While both antibodies recognized vimentin in Sertoli cells, only Mab B2 recognized vimentin in urogenital tract epithelia. Conclusions: Vimentin is expressed in the urogenital tract, and differential reactivities of antibodies may be responsible for conflicting results reported elsewhere for vimentin expression.

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页码：2190 / 2196

页数：7

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