METHODS TO QUANTITATE HUMAN HAPTOGLOBIN BY COMPLEXATION WITH HEMOGLOBIN

被引：14

作者：

CERDA, S ^{[1
]}

OH, SK ^{[1
]}

机构：

[1] BOSTON UNIV,SCH MED,DEPT MICROBIOL,S-413,80 E CONCORD ST,BOSTON,MA 02118

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 134卷 / 01期

关键词：

Affinity chromatography; Monoclonal antibody; ELISA; Haptoglobin; Hemoglobin; Polyclonal antiserum;

D O I：

10.1016/0022-1759(90)90111-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Native human haptoglobin isolated from normal human plasma by affinity chromatography on chicken hemoglobin -Sepharose was used as standard antigen. A direct sandwich ELISA for haptoglobin was developed, with human hemoglobin as a capturing agent. The peroxidase activity of the complex was measured as a means of detecting functional haptoglobin. The reactivities of monoclonal antibody vs. polyclonal antisera on the haptoglobin-hemoglobin complex were compared. Adopting a monoclonal antibody, clone 21.7, which is directed to the α chain of haptoglobin, a specific method to quantitate the native haptoglobin which can complex with hemoglobin has been developed. © 1990.

引用

页码：51 / 59

页数：9

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