METHODS TO QUANTITATE HUMAN HAPTOGLOBIN BY COMPLEXATION WITH HEMOGLOBIN

被引：14

作者：

CERDA, S ^{[1
]}

OH, SK ^{[1
]}

机构：

[1] BOSTON UNIV,SCH MED,DEPT MICROBIOL,S-413,80 E CONCORD ST,BOSTON,MA 02118

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 134卷 / 01期

关键词：

Affinity chromatography; Monoclonal antibody; ELISA; Haptoglobin; Hemoglobin; Polyclonal antiserum;

D O I：

10.1016/0022-1759(90)90111-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Native human haptoglobin isolated from normal human plasma by affinity chromatography on chicken hemoglobin -Sepharose was used as standard antigen. A direct sandwich ELISA for haptoglobin was developed, with human hemoglobin as a capturing agent. The peroxidase activity of the complex was measured as a means of detecting functional haptoglobin. The reactivities of monoclonal antibody vs. polyclonal antisera on the haptoglobin-hemoglobin complex were compared. Adopting a monoclonal antibody, clone 21.7, which is directed to the α chain of haptoglobin, a specific method to quantitate the native haptoglobin which can complex with hemoglobin has been developed. © 1990.

引用

页码：51 / 59

页数：9

共 22 条

[11] SPECIFIC BINDING OF HAPTOGLOBIN TO HUMAN NEUTROPHILS AND ITS FUNCTIONAL CONSEQUENCES [J].

OH, SK ;

PAVLOTSKY, N ;

TAUBER, AI .

JOURNAL OF LEUKOCYTE BIOLOGY, 1990, 47 (02) :142-148

[12] MONOCLONAL-ANTIBODY TO SER IMMUNE SUPPRESSOR DETECTS POLYMERIC FORMS OF HAPTOGLOBIN [J].

OH, SK ;

VERY, DL ;

ETTINGER, R ;

WALKER, J ;

GIAMPAOLO, C ;

BERNARDO, J .

HYBRIDOMA, 1989, 8 (04) :449-466

[13]

OH SK, 1987, CANCER RES, V47, P5120

[14]

OH SK, 1988, IMMUNOLOGY, V64, P73

[15]

OH SK, 1990, IN PRESS J NATL CANC

[16]

PUTMAN FW, 1975, PLASMA PROTEINS STRU, V2, P1

[17] A GENERAL-METHOD FOR THE ISOLATION OF HAPTOGLOBIN 1-1, 2-1, AND 2-2 FROM HUMAN-PLASMA [J].

RADEMACHER, BE ;

STEELE, WJ .

ANALYTICAL BIOCHEMISTRY, 1987, 160 (01) :119-126

[18] IMPROVED PROCEDURE FOR STARCH-GEL ELECTROPHORESIS - FURTHER VARIATIONS IN THE SERUM PROTEINS OF NORMAL INDIVIDUALS [J].

SMITHIES, O .

BIOCHEMICAL JOURNAL, 1959, 71 :585-&

[19] NOTATION FOR SERUM-PROTEIN GROUPS AND THE GENES CONTROLLING THEIR INHERITANCE [J].

SMITHIES, O ;

WALKER, NF .

NATURE, 1956, 178 (4535) :694-695

[20] ELECTROPHORETIC TRANSFER OF PROTEINS FROM POLYACRYLAMIDE GELS TO NITROCELLULOSE SHEETS - PROCEDURE AND SOME APPLICATIONS [J].

TOWBIN, H ;

STAEHELIN, T ;

GORDON, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (09) :4350-4354

← 1 2 3 →