CLONING OF A D-TYPE CYCLIN FROM MURINE ERYTHROLEUKEMIA-CELLS

被引：69

作者：

KIYOKAWA, H ^{[1
]}

BUSQUETS, X ^{[1
]}

POWELL, CT ^{[1
]}

NGO, L ^{[1
]}

RIFKIND, RA ^{[1
]}

MARKS, PA ^{[1
]}

机构：

[1] CORNELL UNIV, GRAD SCH MED SCI, SLOAN KETTERING DIV, NEW YORK, NY 10021 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 06期

关键词：

CYL2; CDNA; CELL CYCLE;

D O I：

10.1073/pnas.89.6.2444

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We report the complete coding sequence of a cDNA, designated CYL2, derived from a murine erythroleukemia cell library. CYL2 is considered to encode a D-type cyclin because (i) there is cross hybridization with CYL1 (a murine homolog of human cyclin D1) and the encoded protein has 64% amino acid sequence identity with CYL1 and (ii) murine erythroleukemia cell-derived CYL2 contains an amino acid sequence identical to that previously reported for the C-terminal portion of a partially sequenced CYL2. Transcripts of murine erythroleukemia cell CYL2 undergo alternative polyadenylylation like that of human cyclin D1. A major 6.5-kilobase CYL2 transcript changes its expression during the cell cycle with a broad peak through G1 and S phases and a decrease in G2/M phases. The present findings suggest that CYL2 plays a role in the G1 to S phase progression.

引用

页码：2444 / 2447

页数：4