EFFECTS OF TEMPERATURE ON VIROGENESIS OF BLUETONGUE VIRUS SEROTYPE-11 IN CULICOIDES-VARIIPENNIS-SONORENSIS

被引：59

作者：

MULLENS, BA ^{[1
]}

TABACHNICK, WJ ^{[1
]}

HOLBROOK, FR ^{[1
]}

THOMPSON, LH ^{[1
]}

机构：

[1] USDA ARS, ARTHROPOD BORNE ANIM DIS RES LAB, LARAMIE, WY USA

来源：

MEDICAL AND VETERINARY ENTOMOLOGY | 1995年 / 9卷 / 01期

关键词：

BLUETONGUE; ARBOVIRUS; TEMPERATURE; DIPTERA; CERATOPOGONIDAE; CULICOIDES; EPIZOOTIOLOGY;

D O I：

10.1111/j.1365-2915.1995.tb00119.x

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Culicoides variipennis sonorensis females were fed bluetongue virus serotype 11 mixed in sheep blood and were held at constant temperatures of 32, 27, 21 and 15-degrees-C. Virogenesis, as measured by enzyme-linked immunosorbent assay (ELISA), proceeded significantly faster at higher temperatures. Based on ELISA absorbance greater-than-or-equal-to 0.2, some flies first were categorized as infected after 1 day, 2 days and 4 days at 32, 27 and 21-degrees-C, respectively. Peak levels of virus antigen were seen after 5-7, 7-13 and 18-22 days for flies held at 32, 27 and 21-degrees-C, respectively. There was no significant virus replication in flies held at 15-degrees-C for 22 days, but latent virus replicated and was detected easily (44% infection) 4-10 days after these flies were transferred to 27-degrees-C. The implications for temperature effects on bluetongue epizootiology are discussed.

引用

页码：71 / 76

页数：6