PITUITARY HYPERPLASIA INDUCED BY ECTOPIC EXPRESSION OF NERVE GROWTH-FACTOR

被引：66

作者：

BORRELLI, E ^{[1
]}

SAWCHENKO, PE ^{[1
]}

EVANS, RM ^{[1
]}

机构：

[1] INSERM,U184,GENET MOLEC EUCARYOTES LAB,F-67000 STRASBOURG,FRANCE

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 07期

关键词：

D O I：

10.1073/pnas.89.7.2764

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Nerve growth factor (NGF) cDNA was fused to the rat prolactin promoter to induce its ectopic expression in pituitary lactotrophs of transgenic mice. High-level expression of both RNA and functional protein was achieved in two pedigrees. Pituitary cells from these animals secreted biologically active NGF that was capable of inducing rapid differentiation of cocultured PC12 pheochromocytoma cells. Despite this robust expression, transgenic pituitaries failed to show any detectable increase in neuronal innervation. Unexpectedly, we observed a dramatic hyperplasia of lactotrophs resulting in pituitaries 10-100 times larger than normal. These results suggest that NGF, in addition to its previously described effects, may act as a new class of mitogen, with a potential role in oncogenesis.

引用

页码：2764 / 2768

页数：5

共 23 条

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