RAPID ENZYME-LINKED-IMMUNOSORBENT-ASSAY WITH FUNCTIONAL MAGNETITE PARTICLES

被引：17

作者：

SHINKAI, M ^{[1
]}

WANG, J ^{[1
]}

KAMIHIRA, M ^{[1
]}

IWATA, M ^{[1
]}

HONDA, H ^{[1
]}

KOBAYASHI, T ^{[1
]}

机构：

[1] NAGOYA UNIV,FAC ENGN,DEPT CHEM ENGN,NAGOYA 46401,JAPAN

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1992年 / 73卷 / 02期

关键词：

D O I：

10.1016/0922-338X(92)90683-L

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Ultrafine magnetite particles were applied to enzyme-linked immunosorbent assay (ELISA). For the assay of mouse IgG, anti-mouse IgG antibody or protein A was immobilized on the magnetite particles. The assay strategy was based on the sandwich method. Using anti-mouse IgG-magnetite and the spectrofluorometry, the mouse IgG standard curve was linear when the IgG concentration was between 5-mu-g/l-5 mg/l. Using these functional magnetite particles, we could shorten the assay time to one thirty-fifth that of the conventional method.

引用

页码：166 / 168

页数：3