FIBEROPTIC SENSOR FOR CONTINUOUS MONITORING OF FERMENTATION PH

被引：24

作者：

AGAYN, VI ^{[1
]}

WALT, DR ^{[1
]}

机构：

[1] TUFTS UNIV,MAX TISHLER LAB ORGAN CHEM,DEPT CHEM,MEDFORD,MA 02155

来源：

BIO-TECHNOLOGY | 1993年 / 11卷 / 06期

关键词：

D O I：

10.1038/nbt0693-726

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have developed a fiber-optic chemical sensor for on-line monitoring of fermentation pH. The sensor is based on a covalently bound fluorescent dye immobilized within a water-permeable polymer layer on an optical fiber. Measurements were performed on a portable fluorimeter and employed a ratiometric approach to account for system instabilities. We show that the use of this fiber-optic sensor provides fast, accurate and reliable measurements during E. coli fermentation in a complex medium.

引用

页码：726 / 729

页数：4

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