MESO-2,5-DIMERCAPTO-N,N,N',N'-TETRAMETHYLADIPAMIDE - A READILY AVAILABLE, KINETICALLY RAPID REAGENT FOR THE REDUCTION OF DISULFIDES IN AQUEOUS-SOLUTION

被引：30

作者：

LEES, WJ ^{[1
]}

SINGH, R ^{[1
]}

WHITESIDES, GM ^{[1
]}

机构：

[1] HARVARD UNIV, DEPT CHEM, CAMBRIDGE, MA 02138 USA

来源：

JOURNAL OF ORGANIC CHEMISTRY | 1991年 / 56卷 / 26期

关键词：

D O I：

10.1021/jo00026a026

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

meso-2,5-Dimercapto-N,N,N',N'-tetramethyladipamide (meso-DTA) reduces disulfide bonds up to 8 times faster (kinetic) than does dithiothreitol (DTT) in aqueous solution at pH 7.0. meso-DTA is easily synthesized in five steps (39% overall yield) from adipic acid. meso-DTA, which forms a cyclic disulfide, is less reducing than DTT by approximately 56 mV, but is much more reducing than mercaptoethanol.

引用

页码：7328 / 7331

页数：4

共 19 条

[11]

PRICE PA, 1969, J BIOL CHEM, V244, P929

[12] RATES OF THIOL-DISULFIDE INTERCHANGE REACTIONS INVOLVING PROTEINS AND KINETIC MEASUREMENTS OF THIOL PKA VALUES [J].