GENE-EXPRESSION AND TRANSCRIPT SIZE OF THE PREPRO-PEPTIDE VIP/PHM-27 IN NORMAL HUMAN TISSUE

被引:6
作者
SCHULTEBOCKHOLT, A
MEIER, DA
HOPP, K
STADELMANN, A
OTTERSON, MF
TELFORD, GL
KOCH, TR
机构
[1] ZABLOCKI VET ADM MED CTR,DIV ENDOCRINOL,MILWAUKEE,WI 53295
[2] ZABLOCKI VET ADM MED CTR,DIV SURG,MILWAUKEE,WI 53295
[3] MED COLL WISCONSIN,DIGEST DIS RES CTR,MILWAUKEE,WI 53295
关键词
VASOACTIVE INTESTINAL PEPTIDE; PEPTIDE HISTIDINE METHIONINE 27; TRANSCRIPT SIZE; GENE EXPRESSION; HUMAN TISSUE; NEUROPEPTIDE;
D O I
10.1016/0304-3940(93)90867-K
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The transcript size of VIP/PHM-27 mRNA (vasoactive intestinal peptide/peptide histidine methionine) and the relative distribution of VIP/PHM-27 gene expression in 10 normal human tissues was examined. After mRNA extraction from tissue, VIP/PHM-27 transcript size and relative abundance of mRNA was determined by Northern blot analysis and densitometry of the autoradiograms. VIP/PHM-27 mRNA was detectable in brain, pancreas, colon, ileum and striated muscle while no hybridization signal was observed in liver, kidney, lung, heart, prostate and placental tissue. VIP/PHM-27 transcript in human brain and gut was a single band of 1.7 kb; by contrast, a 7.0-kb transcript was detected in striated skeletal muscle. The highest relative levels of mRNA were observed in brain and pancreas.
引用
收藏
页码:101 / 104
页数:4
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