GENE-EXPRESSION AND TRANSCRIPT SIZE OF THE PREPRO-PEPTIDE VIP/PHM-27 IN NORMAL HUMAN TISSUE

被引：6

作者：

SCHULTEBOCKHOLT, A

MEIER, DA

HOPP, K

STADELMANN, A

OTTERSON, MF

TELFORD, GL

KOCH, TR

机构：

[1] ZABLOCKI VET ADM MED CTR,DIV ENDOCRINOL,MILWAUKEE,WI 53295

[2] ZABLOCKI VET ADM MED CTR,DIV SURG,MILWAUKEE,WI 53295

[3] MED COLL WISCONSIN,DIGEST DIS RES CTR,MILWAUKEE,WI 53295

来源：

NEUROSCIENCE LETTERS | 1993年 / 164卷 / 1-2期

关键词：

VASOACTIVE INTESTINAL PEPTIDE; PEPTIDE HISTIDINE METHIONINE 27; TRANSCRIPT SIZE; GENE EXPRESSION; HUMAN TISSUE; NEUROPEPTIDE;

D O I：

10.1016/0304-3940(93)90867-K

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The transcript size of VIP/PHM-27 mRNA (vasoactive intestinal peptide/peptide histidine methionine) and the relative distribution of VIP/PHM-27 gene expression in 10 normal human tissues was examined. After mRNA extraction from tissue, VIP/PHM-27 transcript size and relative abundance of mRNA was determined by Northern blot analysis and densitometry of the autoradiograms. VIP/PHM-27 mRNA was detectable in brain, pancreas, colon, ileum and striated muscle while no hybridization signal was observed in liver, kidney, lung, heart, prostate and placental tissue. VIP/PHM-27 transcript in human brain and gut was a single band of 1.7 kb; by contrast, a 7.0-kb transcript was detected in striated skeletal muscle. The highest relative levels of mRNA were observed in brain and pancreas.

引用

页码：101 / 104

页数：4

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